Comparative mitogenomic analysis of the superfamily Pentatomoidea Insecta: Hemiptera: Heteroptera and phylogenetic implicationsReport as inadecuate




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BMC Genomics

, 16:460

First Online: 16 June 2015Received: 04 December 2014Accepted: 01 June 2015DOI: 10.1186-s12864-015-1679-x

Cite this article as: Yuan, ML., Zhang, QL., Guo, ZL. et al. BMC Genomics 2015 16: 460. doi:10.1186-s12864-015-1679-x

Abstract

BackgroundInsect mitochondrial genomes mitogenomes are the most extensively used genetic marker for evolutionary and population genetics studies of insects. The Pentatomoidea superfamily is economically important and the largest superfamily within Pentatomomorpha with over 7,000 species. To better understand the diversity and evolution of pentatomoid species, we sequenced and annotated the mitogenomes of Eurydema gebleri and Rubiconia intermedia, and present the first comparative analysis of the 11 pentatomoid mitogenomes that have been sequenced to date.

ResultsWe obtained the complete mitogenome of Eurydema gebleri 16,005 bp and a nearly complete mitogenome of Rubiconia intermedia 14,967 bp. Our results show that gene content, gene arrangement, base composition, codon usage, and mitochondrial transcription termination factor sequences are highly conserved in pentatomoid species, especially for species in the same family. Evolutionary rate analyses of protein-coding genes reveal that the highest and lowest rates are found in atp8 and cox1 and distinctive evolutionary patterns are significantly correlated with the G + C content of genes. We inferred the secondary structures for two rRNA genes for eleven pentatomoid species, and identify some conserved motifs of RNA structures in Pentatomidea. All tRNA genes in pentatomoid mitogenomes have a canonical cloverleaf secondary structure, except for two tRNAs trnS1 and trnV which appear to lack the dihydrouridine arm. Regions that are A + T-rich have several distinct characteristics e.g. size variation and abundant tandem repeats, and have potential as species or population level molecular markers. Phylogenetic analyses based on mitogenomic data strongly support the monophyly of Pentatomoidea, and the estimated phylogenetic relationships are: Urostylididae + Plataspidae + Pentatomidae + Cydnidae + Dinidoridae + Tessaratomidae.

ConclusionsThis comparative mitogenomic analysis sheds light on the architecture and evolution of mitogenomes in the superfamily Pentatomoidea. Mitogenomes can be effectively used to resolve phylogenetic relationships of pentatomomorphan insects at various taxonomic levels. Sequencing more mitogenomes at various taxonomic levels, particularly from closely related species, will improve the annotation accuracy of mitochondrial genes, as well as greatly enhance our understanding of mitogenomic evolution and phylogenetic relationships in pentatomoids.

AbbreviationsPCGsProtein-coding genes

atp6 and atp8Genes for the ATPase subunits 6 and 8

cox1-cox3Genes for cytochrome C oxidase subunits I-III

cobA gene for apocytochrome b

nad1-nad6 and nad4LGenes for NADH dehydrogenase subunits 1–6 and 4 L

rrnLLarge 16S rRNA subunit gene

rrnSSmall 12S rRNA subunit gene

trnX where X is replaced by one letter amino acid code of the corresponding amino acidTransfer RNA

Ming-Long Yuan and Qi-Lin Zhang contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-1679-x contains supplementary material, which is available to authorized users.

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Author: Ming-Long Yuan - Qi-Lin Zhang - Zhong-Long Guo - Juan Wang - Yu-Ying Shen

Source: https://link.springer.com/



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