Genetic analysis reveals diversity and genetic relationship among Trichoderma isolates from potting media, cultivated soil and uncultivated soilReport as inadecuate




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BMC Microbiology

, 15:147

Ecological and evolutionary microbiology

Abstract

BackgroundTrichoderma is one of the most common fungi in soil. However, little information is available concerning the diversity of Trichoderma in soil with no previous history of cultivation. This study was conducted to investigate the most common species and the level of genetic relatedness of Trichoderma species from uncultivated soil in relation to cultivated soil and potting media.

ResultsA total of 24, 15 and 13 Trichoderma isolates were recovered from 84 potting media samples, 45 cultivated soil samples and 65 uncultivated soil samples, respectively. Analysis based on the internal transcribed spacer region of the ribosomal RNA rRNA and the translation elongation factor gene EF1 indicated the presence of 9 Trichoderma species: T. harzianum 16 isolates, T. asperellum 13, T. citrinoviride 9, T. orientalis 3, T. ghanense 3, T. hamatum 3, T. longibrachiatum 2, T. atroviride 2, and T. viride 1. All species were found to occur in potting media samples, while five Trichoderma species were recovered from the cultivated soils and four from the uncultivated soils. AFLP analysis of the 52 Trichoderma isolates produced 52 genotypes and 993 polymorphic loci. Low to moderate levels of genetic diversity were found within populations of Trichoderma species H = 0.0780 to 0.2208. Analysis of Molecular Variance indicated the presence of very low levels of genetic differentiation Fst = 0.0002 to 0.0139 among populations of the same Trichoderma species obtained from the potting media, cultivated soil and uncultivated soil.

ConclusionThe study provides evidence for occurrence of Trichoderma isolates in soil with no previous history of cultivation. The lack of genetic differentiation among Trichoderma populations from potting media, cultivated soil and uncultivated soil suggests that some factors could have been responsible for moving Trichoderma propagules among the three substrates. The study reports for the first time the presence of 4 Trichoderma species in Oman: T. asperellum, T. ghanense, T. longibrachiatum and T. orientalis.

KeywordsBiological control Fallow soil Phylogenetic analysis  Download fulltext PDF



Author: Abdullah M. Al-Sadi - Fatma A. Al-Oweisi - Simon G. Edwards - Hamed Al-Nadabi - Ahmed M. Al-Fahdi

Source: https://link.springer.com/







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