Carbapenem-resistant and carbapenem-susceptible isogenic isolates of Klebsiella pneumoniae ST101 causing infection in a tertiary hospitalReport as inadecuate




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BMC Microbiology

, 15:177

Clinical microbiology and vaccines

Abstract

BackgroundIn this study we describe the clinical and molecular characteristics of an outbreak due to carbapenem-resistant Klebsiella pneumoniae CR-KP producing CTX-M-15 and OXA-48 carbapenemase. Isogenic strains, carbapenem-susceptible K. pneumoniae CS-KP producing CTX-M-15, were also involved in the outbreak.

ResultsFrom October 2010 to December 2012 a total of 62 CR-KP and 23 CS-KP were isolated from clinical samples of 42 patients 22 had resistant isolates, 14 had susceptible isolates, and 6 had both CR and CS isolates. All patients had underlying diseases and 17 of them 14 patients with CR-KP and 3 with CS-KP had received carbapenems previously. The range of carbapenem MICs for total isolates were: imipenem: 2 to >32 μg-ml vs. <2 μg-ml; meropenem: 4 to >32 μg-ml vs. <2 μg-ml; and ertapenem: 8 to >32 μg-ml vs. <2 μg-ml. All the isolates were also resistant to gentamicin, ciprofloxacin, and cotrimoxazole. Both types of isolates shared a common PFGE pattern associated with the multilocus sequence type 101 ST101. The blaCTX-M-15 gene was detected in all the isolates, whereas the blaOXA-48 gene was only detected in CR-KP isolates on a 70 kb plasmid.

ConclusionsThe clonal spread of K. pneumoniae ST101 expressing the OXA-48 and CTX-M-15 beta-lactamases was the cause of an outbreak of CR-KP infections. CTX-M-15-producing isolates lacking the blaOXA-48 gene coexisted during the outbreak.

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Author: Meritxell Cubero - Guillermo Cuervo - M. Ángeles Dominguez - Fe Tubau - Sara Martí - Elena Sevillano - Lucía Gallego -

Source: https://link.springer.com/







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