Intraoperative Sentinel Lymph Node Evaluation: Implications of Cytokeratin 19 Expression for the Adoption of OSNA in Oral Squamous Cell CarcinomaReport as inadecuate




Intraoperative Sentinel Lymph Node Evaluation: Implications of Cytokeratin 19 Expression for the Adoption of OSNA in Oral Squamous Cell Carcinoma - Download this document for free, or read online. Document in PDF available to download.

Annals of Surgical Oncology

, Volume 23, Issue 12, pp 4042–4048

First Online: 08 July 2016Received: 26 March 2016DOI: 10.1245-s10434-016-5337-6

Cite this article as: Shaw, R., Christensen, A., Java, K. et al. Ann Surg Oncol 2016 23: 4042. doi:10.1245-s10434-016-5337-6

Abstract

BackgroundIntraoperative analysis of sentinel lymph nodes would enhance the care of early-stage oral squamous cell carcinoma OSCC. We determined the frequency and extent of cytokeratin 19 CK19 expression in OSCC primary tumours and surrounding tissues to explore the feasibility of a -clinic-ready- intraoperative diagnostic test one step nucleic acid amplification—OSNA, sysmex.

MethodsTwo cohorts were assembled: cohort 1, OSCC with stage and site that closely match cases suitable for sentinel lymph node biopsy SLNB; cohort 2, HNSCC with sufficient fresh tumour tissue available for the OSNA assay >50 mg. CK19 assays included qRT-PCR, RNA in situ hybridisation ISH, and immunohistochemistry IHC, as well as OSNA.

ResultsCK19 mRNA expression was detected with variable sensitivity, depending on method, in 60–80% of primary OSCC tumours, while protein expression was observed in only 50% of tumours. Discordance between different techniques indicated that OSNA was more sensitive than qRT-PCR or RNA-ISH, which in turn were more sensitive than IHC. OSNA results showed CK19 expression in 80% of primary cases, so if used for diagnosis of lymph node metastasis would lead to a false-negative result in 20% of patients with cervical lymph node metastases.

ConclusionsOSNA in its current form is not suitable for use in OSCC SLNB due to inadequate expression of the CK19 target in all case. However, the same assay technology would likely be very promising if applied using a more ubiquitous squamous epithelial target.

Richard Shaw and Anders Christensen are joint first authors. Janet Risk and Max Robinson are joint senior authors.

Electronic supplementary materialThe online version of this article doi:10.1245-s10434-016-5337-6 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Richard Shaw - Anders Christensen - Kapil Java - Rehab El Maddani - Triantafillos Liloglou - Triantafyllou Asterios - Chris

Source: https://link.springer.com/







Related documents