Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritisReport as inadecuate




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Biological Research

, 49:17

First Online: 11 March 2016Received: 08 October 2015Accepted: 02 March 2016DOI: 10.1186-s40659-016-0078-3

Cite this article as: Geng, W., Wei, R., Liu, S. et al. Biol Res 2016 49: 17. doi:10.1186-s40659-016-0078-3

Abstract

BackgroundIn China, mesangial proliferative glomerulonephritis MsPGN is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: 1 Sham surgery Sham; 2 anti-Thy-1 nephritis model Thy-1; 3 anti-Thy-1 nephritis model + irbesartan-treated Irb; 4 anti-Thy-1 nephritis model + low-dose of Shenhua Tablet SHL; 5 anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet SHM; 6 anti-Thy-1 nephritis model + high-dose of Shenhua Tablet SHH.

ResultsThirteen weeks after drug treatment, urinary proteins were quantified and renal pathological changes were thoroughly examined at the time point of 24 h. Meanwhile, the expression levels of p-Erk1-2, cyclin D1 and p21 at the renal cortex were also tested. The levels of urinary proteins and total cholesterol in the blood were significantly reduced in rats treated with any drug tested in this study. The level of triglyceride was significantly reduced in all three Shenhua Tablet-treated groups. Renal pathomorphological scores were significantly improved in groups of Irb, SHM and SHH. Mesangial cell proliferation was significantly inhibited in any drug-treated group. p-Erk1-2 and cyclin D1 were downregulated whereas p21 was upregulated in the renal cortex.

ConclusionsOur study indicated that Shenhua Tablet is able to inhibit the abnormal proliferation of mesangial cells and to prevent kidney damage, which is likely associated with downregulation of p-Erk1-2 and reduced activity of its downstream target-cyclin D1.

KeywordsShenhua Tablet Chronic anti-Thy-1 glomerulonephritis Mesangial cell proliferation Erk AbbreviationsMsPGNmesangial proliferative glomerulonephritis

Irbirbesartan

ESRDend-stage renal disease

PCNAproliferating cell nuclear antigen

TLRstoll-like receptors

SHLlow-dose of Shenhua Tablet

SHMmedium-dose of Shenhua Tablet

SHHhigh-dose of Shenhua Tablet

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Author: Wenjia Geng - Ribao Wei - Shuwen Liu - Li Tang - Hanyu Zhu - Pu Chen - Jie Wu - Xueguang Zhang - Fei Zhu - Zhong Yin - X

Source: https://link.springer.com/







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