Characterization of the anti-inflammatory Lactobacillus reuteri BM36301 and its probiotic benefits on aged miceReport as inadecuate




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BMC Microbiology

, 16:69

Applied microbiology

Abstract

BackgroundThe gut microbiota is playing more important roles in host immune regulation than was initially expected. Since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest.

ResultsWe have screened our collection of probiotic lactic acid bacteria LAB for their efficacy in modulating host immune response. Some LAB are characterized by suppression of TNF-α induction when LAB culture supernatants are added to THP-1 cells, demonstrating the LAB’s anti-inflammatory potential. These suppressive materials were not inactivated by heat or trypsin. On the other hand, treatment of THP-1 directly with live bacterial cells identified a group of pro-inflammatory LAB, which stimulated significant production of TNF-α. Among those, we chose the Lactobacillus reuteri BM36301 as an anti-inflammatory strain and the L. reuteri BM36304 as a pro-inflammatory strain, and further studied their in vivo effects. We supplied C57BL-6 mice with these bacteria in drinking water while feeding them a standard diet for 20 weeks. Interestingly, these L. reuteri strains evoked different consequences depending on the gender of the mice. That is, males treated with anti-inflammatory BM36301 experienced less weight gain and higher testosterone level; females treated with BM36301 maintained lower serum TNF-α as well as healthy skin with active folliculogenesis and hair growth. Furthermore, while males treated with pro-inflammatory BM36304 developed higher serum levels of TNF-α and insulin, in contrast females did not experience such effects from this bacteria strain.

ConclusionThe L. reuteri BM36301 was selected as an anti-inflammatory strain in vitro. It helped mice maintain healthy conditions as they aged. These findings propose the L. reuteri BM36301 as a potential probiotic strain to improve various aspects of aging issues.

KeywordsProbiotics L. reuteri Lactic acid bacteria Anti-inflammatory TNF-α C57BL-6 mice Aging Testosterone Skin health Hair growth AbbreviationsBMBenebios Microorganism

CMconditioned medium

ELISAenzyme-linked immunosorbent assay

HFhair follicle

IBDinflammatory bowel disease

IECintestinal epithelial cell

ILinterleukin

LABlactic acid bacteria

LPSlipopolysaccharide

TLRtoll-like receptor

TNFtumor necrosis factor

Electronic supplementary materialThe online version of this article doi:10.1186-s12866-016-0686-7 contains supplementary material, which is available to authorized users.

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Author: Joon Lee - Woo Yang - Andrew Hostetler - Nathan Schultz - Mark A. Suckow - Kay L. Stewart - Daniel D. Kim - Hyung Soo 

Source: https://link.springer.com/







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