Severe compromise of preosteoblasts in a surgical mouse model of bisphosphonate-associated osteonecrosis of the jawReport as inadecuate




Severe compromise of preosteoblasts in a surgical mouse model of bisphosphonate-associated osteonecrosis of the jaw - Download this document for free, or read online. Document in PDF available to download.

Resumen

Objectives: The effect of amino-bisphosphonates on osteoblastic lineage and its potential contribution to the pathogenesis of bisphosphonate-associated osteonecrosis of the jaw BONJ remain controversial. We assessed the effects of zoledronic acid ZOL on bone and vascular cells of the alveolar socket using a mouse model of BONJ. Material and methods: Thirty-two mice were treated twice a week with either 100 mu g-kg of ZOL or saline for 12 weeks. The first left maxillary molar was extracted at the third week. Alveolar sockets were assessed at both 3 weeks intermediate and 9 weeks long-term after molar extraction by semi quantitative histomorphometry for empty lacunae, preosteoblasts Osterix, osteoclasts TRAP, and pericyte-like cells CD146. Also, the bone microarchitecture was assessed by micro-CT. Results: Osteonecrotic-like lesions were observed in 21% of mice. Moreover, a decreased number of preosteoblasts contrasted with the increased number of osteoclasts at both time points. In addition, osteoclasts display multinucleation and detachment from the endosteal surface. Furthermore, the number of pericyte-like cells increased at the intermediate time point. The alveolar bone mass increased exclusively with long-term ZOL treatment. Conclusion: The severe imbalance between bone-forming cells and bone-resorbing cells shown in this study could contribute to the pathogenesis of BONJ.Indización

Artículo de publicación ISI



Author: Córdova Jara, Luis Alberto; - Guilbaud, Florian; - Amiaud, Jerome; - Battaglia, Severine; - Charrier, Celine; - Lezot, Frederic;

Source: http://repositorio.uchile.cl/



DOWNLOAD PDF




Related documents