Identification of Salmonella enterica serovar Kentucky genes involved in attachment to chicken skinReport as inadecuate




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BMC Microbiology

, 16:168

Microbe-host interactions and microbial pathogenicity

Abstract

BackgroundRegardless of sanitation practices implemented to reduce Salmonella prevalence in poultry processing plants, the problem continues to be an issue. To gain an understanding of the attachment mechanism of Salmonella to broiler skin, a bioluminescent-based mutant screening assay was used. A random mutant library of a field-isolated bioluminescent strain of Salmonella enterica serovar Kentucky was constructed. Mutants’ attachment to chicken skin was assessed in 96-well plates containing uniform 6 mm diameter pieces of circular chicken skin. After washing steps, mutants with reduced attachment were selected based on reduced bioluminescence, and transposon insertion sites were identified.

ResultsAttachment attenuation was detected in transposon mutants with insertion in genes encoding flagella biosynthesis, lipopolysaccharide core biosynthesis protein, tryptophan biosynthesis, amino acid catabolism pathway, shikimate pathway, tricarboxylic acid TCA cycle, conjugative transfer system, multidrug resistant protein, and ATP-binding cassette ABC transporter system. In particular, mutations in S. Kentucky flagellar biosynthesis genes flgA, flgC, flgK, flhB, and flgJ led to the poorest attachment of the bacterium to skin.

ConclusionsThe current study indicates that attachment of Salmonella to broiler skin is a multifactorial process, in which flagella play an important role.

KeywordsSalmonella Kentucky Flagella Poultry Skin Attachment  Download fulltext PDF



Author: Sanaz Salehi - Kevin Howe - John Brooks - Mark L. Lawrence - R. Hartford Bailey - Attila Karsi

Source: https://link.springer.com/







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