The gene-expression profile of renal medulla in ISIAH rats with inherited stress-induced arterial hypertensionReport as inadecuate




The gene-expression profile of renal medulla in ISIAH rats with inherited stress-induced arterial hypertension - Download this document for free, or read online. Document in PDF available to download.

BMC Genetics

, 17:151

First Online: 22 December 2016DOI: 10.1186-s12863-016-0462-6

Cite this article as: Ryazanova, M.A., Fedoseeva, L.A., Ershov, N.I. et al. BMC Genet 2016 17Suppl 3: 151. doi:10.1186-s12863-016-0462-6

Abstract

BackgroundThe changes in the renal function leading to a reduction of medullary blood flow can have a great impact on sodium and water homeostasis and on the long-term control of arterial blood pressure. The RNA-Seq approach was used for transcriptome profiling of the renal medulla from hypertensive ISIAH and normotensive WAG rats to uncover the genetic basis of the changes underlying the renal medulla function in the ISIAH rats being a model of the stress-sensitive arterial hypertension and to reveal the genes which possibly may contribute to the alterations in medullary blood flow.

ResultsMultiple DEGs specifying the function of renal medulla in ISIAH rats were revealed. The group of DEGs described by Gene Ontology term ‘oxidation reduction’ was the most significantly enriched one. The other groups of DEGs related to response to external stimulus, response to hormone endogenous stimulus, response to stress, and homeostatic process provide the molecular basis for integrated responses to homeostasis disturbances in the renal medulla of the ISIAH rats. Several DEGs, which may modulate the renal medulla blood flow, were detected. The reduced transcription of Nos3 pointed to the possible reduction of the blood flow in the renal medulla of ISIAH rats.

ConclusionsThe generated data may be useful for comparison with those from different models of hypertension and for identifying the common molecular determinants contributing to disease manifestation, which may be potentially used as new pharmacological targets.

KeywordsStress-sensitive hypertension Renal medulla Transcriptional profiling RNA-Seq ISIAH rats Abbreviations20-HETE20-hydroxyeicosatetraenoic acid

BPBlood pressure

DAVIDDatabase for Annotation, Visualization and Integrated Discovery

DEGsDifferentially expressed genes

EETsEpoxyeicosatrienoic acids

eNOS or NOS3endothelial nitric oxide synthase

FPKMFragments per kilobase of transcript per million mapped reads

GOGene Ontology

ISIAHInherited Stress-Induced Arterial Hypertension

KEGGKyoto Encyclopedia of Genes and Genomes Pathway Database

NONitric oxide

PLS-DAPartial-least squares discriminant analysis

qPCRquantitative real time polymerase chain reaction

RAASRenin-angiotensin-aldosterone system

RGDRat Genome Database

RNA-seqRNA sequencing

ROSReactive oxygen species

SABPSystolic arterial blood pressure

sEHsoluble epoxide hydrolase

SHRSpontaneously hypertensive rats

SNSSympathetic nervous system

SODsSuperoxide dismutases

WAGWistar Albino Glaxo rats

Electronic supplementary materialThe online version of this article doi:10.1186-s12863-016-0462-6 contains supplementary material, which is available to authorized users.

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Author: Marina A. Ryazanova - Larisa A. Fedoseeva - Nikita I. Ershov - Vadim M. Efimov - Arcady L. Markel - Olga E. Redina

Source: https://link.springer.com/



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