Increase in endogenous estradiol in the progeny of obese rats is associated with precocious puberty and altered follicular development in adulthoodReport as inadecuate




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Maternal obesity during pregnancy has beenrelated with several pathological states in offspring.However, the impact of maternal obesity on reproductivesystem on the progeny is beginning to be elucidated. In thiswork, we characterize the effect of maternal obesity onpuberty onset and follicular development in adult offspringin rats. We also propose that alterations in ovarian physiologyobserved in offspring of obese mothers are due toincreased levels of estradiol during early development.Offspring of control dams and offspring of dams exposedto a high-fat diet HF were studied at postnatal daysPND 1, 7, 14, 30, 60, and 120. Body weight and onset ofpuberty were measured. Counting of ovarian follicles wasperformed at PND 60 and 120. Serum estradiol, estriol,androstenedione, FSH, LH, and insulin levels weremeasured by ELISA. Hepatic CYP3A2 expression wasdetermined by Western blot. HF rats had a higher weightthan controls at all ages and they also had a precociouspuberty. Estradiol levels were increased while CYP3A2expression was reduced from PND 1 until PND 60 in HFrats compared to controls. Estriol was decreased at PND60in HF rats. Ovaries from HF rats had a decrease in antralfollicles at PND60 and PND120 and an increase in follicularcysts at PND60 and PND120. In this work, wedemonstrated that maternal obesity in rats alters folliculardevelopment and induces follicular cysts generation in theadult offspring. We observed that maternal obesity producesan endocrine disruption through increasing endogenousestradiol in early life. A programmed failure inhepatic metabolism of estradiol is probably the cause of itsincrease.Indización

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Author: Ambrosetti, Valery; - Guerra, Marcelo; - Ramírez, Luisa A.; - Reyes, Aldo; - Álvarez, Daniela; - OlguÍn, SofÍa; - Gonzálz Ma

Source: http://repositorio.uchile.cl/



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