Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine DeficiencyReport as inadecuate




Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2014 2014, Article ID 572915, 9 pages -

Research Article

Department of Geriatrics, Shanghai First People’s Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, China

Department of Neurology, Zhongshan Hospital Affiliated Fudan University, 180 Fenglin Road, Shanghai 200032, China

Received 29 January 2014; Revised 12 April 2014; Accepted 18 May 2014; Published 16 June 2014

Academic Editor: Antoni Camins

Copyright © 2014 Yanling Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells HPCs and the potential mechanisms involved in this effect, we cultured HPCs in vitro in the absence of thiamine and found that proliferation and transketolase activity were both significantly repressed. Furthermore, specific inhibition of transketolase activity by oxythiamine strongly inhibited HPC proliferation in a dose-dependent manner. However, thiamine deficiency itself inhibited the proliferation to a greater degree than did oxythiamine. Taken together, our results suggest that modulation of transketolase activity might be one of the mechanisms by which thiamine regulates the proliferation of hippocampal progenitor cells.





Author: Yanling Zhao, Yiying Wu, Haolu Hu, Jinghui Cai, Min Ning, Xiushi Ni, and Chunjiu Zhong

Source: https://www.hindawi.com/



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