Proteomic approach used in the diagnosis of Riedels thyroiditis: a case reportReport as inadecuate




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Journal of Medical Case Reports

, 6:103

First Online: 05 April 2012Received: 17 November 2011Accepted: 05 April 2012DOI: 10.1186-1752-1947-6-103

Cite this article as: Iacconi, P., Giusti, L., Da Valle, Y. et al. J Med Case Reports 2012 6: 103. doi:10.1186-1752-1947-6-103

Abstract

IntroductionRiedel-s thyroiditis, a rare thyroid disease, can be difficult to diagnose prior to surgical removal and can be confused with malignancy both clinically and cytologically.

Case presentationWe report the case of a 72-year-old Caucasian woman who presented with a goiter, which showed a rapid increase in size at ultrasound check, suggesting malignancy. Because of inconclusive cytology, a total thyroidectomy was performed. Fine-needle aspiration of the removed thyroid was processed by two-dimensional electrophoresis, and the proteome was compared with both anaplastic cancer and control samples. Significant differentially expressed protein spots were identified by Western blot analysis by using specific antibodies.

ConclusionsThe protein pattern of Riedel-s fine-needle aspiration revealed a superimposition with that of the control samples. The comparison of the protein pattern of Riedel-s thyroiditis fine-needle aspiration with that of anaplastic cancer showed evidence of a different expression of ferritin heavy chains, ferritin light chains, and haptoglobins, as previously reported in thyroid cancers. Therefore, we performed Western blot analysis of these proteins and validated that their expression levels were low or absent in Riedel-s thyroiditis and control samples despite the high concentrations present in fine-needle aspiration anaplastic samples. The concurrent absent or low expression levels of haptoglobin, ferritin light chain, and ferritin heavy chain in Riedel-s thyroiditis fine-needle aspiration samples strongly indicate the benign nature of the thyroid lesion. These results suggest the potential applicability of fine-needle aspiration proteome analysis for Riedel-s thyroiditis diagnosis.

Abbreviations2-DEtwo-dimensional electrophoresis

FNAfine-needle aspiration

WBWestern blot.

Electronic supplementary materialThe online version of this article doi:10.1186-1752-1947-6-103 contains supplementary material, which is available to authorized users.

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Author: Pietro Iacconi - Laura Giusti - Ylenia Da Valle - Federica Ciregia - Gino Giannaccini - Liborio Torregrossa - Agnese Proiet

Source: https://link.springer.com/



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