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Environmental Health

, 9:48

First Online: 09 August 2010Received: 21 December 2009Accepted: 09 August 2010DOI: 10.1186-1476-069X-9-48

Cite this article as: Hou, L., Zhu, ZZ., Zhang, X. et al. Environ Health 2010 9: 48. doi:10.1186-1476-069X-9-48

Abstract

BackgroundOxidative stress generation is a primary mechanism mediating the effects of Particulate Matter PM on human health. Although mitochondria are both the major intracellular source and target of oxidative stress, the effect of PM on mitochondria has never been evaluated in exposed individuals.

MethodsIn 63 male healthy steel workers from Brescia, Italy, studied between April and May 2006, we evaluated whether exposure to PM was associated with increased mitochondrial DNA copy number MtDNAcn, an established marker of mitochondria damage and malfunctioning. Relative MtDNAcn RMtDNAcn was determined by real-time PCR in blood DNA obtained on the 1 time 1 and 4 day time 2 of the same work week. Individual exposures to PM10, PM1, coarse particles PM10-PM1 and airborne metal components of PM10 chromium, lead, arsenic, nickel, manganese were estimated based on measurements in the 11 work areas and time spent by the study subjects in each area.

ResultsRMtDNAcn was higher on the 4 day mean = 1.31; 95%CI = 1.22 to 1.40 than on the 1 day of the work week mean = 1.09; 95%CI = 1.00 to 1.17. PM exposure was positively associated with RMtDNAcn on either the 4 PM10: β = 0.06, 95%CI = -0.06 to 0.17; PM1: β = 0.08, 95%CI = -0.08 to 0.23; coarse: β = 0.06, 95%CI = -0.06 to 0.17 or the 1 day PM10: β = 0.18, 95%CI = 0.09 to 0.26; PM1: β = 0.23, 95%CI = 0.11 to 0.35; coarse: β = 0.17, 95%CI = 0.09 to 0.26. Metal concentrations were not associated with RMtDNAcn.

ConclusionsPM exposure is associated with damaged mitochondria, as reflected in increased MtDNAcn. Damaged mitochondria may intensify oxidative-stress production and effects.

List of AbbreviationsBMIbody mass index

MTDNAmitochondrial DNA

MTDNACNmitochondrial DNA copy number

RMTDNACNrelative mitochondrial DNA copy number

PCRpolymerase chain reaction

PMparticulate matter

ROSreactive oxygen species.

Electronic supplementary materialThe online version of this article doi:10.1186-1476-069X-9-48 contains supplementary material, which is available to authorized users.

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Author: Lifang Hou - Zhong-Zheng Zhu - Xiao Zhang - Francesco Nordio - Matteo Bonzini - Joel Schwartz - Mirjam Hoxha - Laura Dioni

Source: https://link.springer.com/







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