Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy SectionsReport as inadecuate




Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections - Download this document for free, or read online. Document in PDF available to download.

Analytical Cellular Pathology - Volume 15 1997, Issue 3, Pages 175-181



Second Department of Pathology, Nagasaki University School of Medicine, Nagasaki, Japan

Third Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan

Second Department of Medicine, Nagasaki University School of Medicine, Nagasaki, Japan



Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Although the role of extracellular matrices in the development of glomerulosclerosis has been discussed widely, the cellular origin of type VI collagen in diabetic nephropathy DN has remained relatively unexplored. This study reports the distribution and cellular origin of type VI collagen in DN. Type VI collagen‐specific oligonucleotide probes and monoclonal antibody were used to assess the relative expression of mRNA for \alpha1 VI chain and its translated protein in paraffin‐embedded renal biopsy sections of DN. By immunohistochemistry, compared to the control, increased deposition of type VI collagen was noted in the diffuse and nodular lesions of diabetic glomeruli. For cellular localization of type VI collagen mRNA, paraffin‐embedded renal sections of the control and DN were hybridized in situ with digoxigenin Dig‐labeled antisense oligo‐DNA probe complementary to a part of \alpha1 VI mRNA. In comparison to the control kidney sections, increased numbers of intraglomerular cells both mesangial and epithelial cells were positive for α1 VI mRNA in renal biopsy sections of DN. From the results, we conclude that overexpression of type VI collagen by intraglomerular cells with its increased deposition might significantly contribute to the glomerulosclerosis found in DN.





Author: Mohammed Shawkat Razzaque, Takehiko Koji, Takashi Harada, and Takashi Taguchi

Source: https://www.hindawi.com/



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