Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine ProductionReport as inadecuate




Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production - Download this document for free, or read online. Document in PDF available to download.

Clinical and Developmental ImmunologyVolume 2012 2012, Article ID 721817, 13 pages

Research Article

Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-000 São Paulo, SP, Brazil

Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY 10065, USA

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, 04023-900 São Paulo, SP, Brazil

Instituto Gulbenkian de Ciência, 2780-901 Oeiras, Portugal

Departamento de Ciências Biológicas, Campus Diadema e Centro de Terapia Celular e Molecular CTCMol, Universidade Federal de São Paulo, 04044-010 São Paulo, SP, Brazil

Instituto Israelita de Ensino e Pesquisa Albert Einstein, 05652-900 São Paulo, SP, Brazil

Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil

Received 13 May 2011; Accepted 31 August 2011

Academic Editor: Valerie Verhasselt

Copyright © 2012 Lucas Faustino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Foxp3

CD25

CD4

regulatory T cells are vital for peripheral tolerance and control of tissue inflammation. In this study, we characterized the phenotype and monitored the migration and activity of regulatory T cells present in the airways of allergic or tolerant mice after allergen challenge. To induce lung allergic inflammation, mice were sensitized twice with ovalbumin-aluminum hydroxide gel and challenged twice with intranasal ovalbumin. Tolerance was induced by oral administration of ovalbumin for 5 consecutive days prior to OVA sensitization and challenge. We detected regulatory T cells Foxp3

CD25

CD4

T cells in the airways of allergic and tolerant mice; however, the number of regulatory T cells was more than 40-fold higher in allergic mice than in tolerant mice. Lung regulatory T cells expressed an effector-memory phenotype CCR4

CD62L

CD44

CD54

CD69

that distinguished them from naive regulatory T cells CCR4

CD62L

CD44

CD54

CD69

. These regulatory T cells efficiently suppressed pulmonary T-cell proliferation but not Th2 cytokine production.





Author: Lucas Faustino, Daniel Mucida, Alexandre Castro Keller, Jocelyne Demengeot, Karina Bortoluci, Luiz Roberto Sardinha, Maisa C

Source: https://www.hindawi.com/



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