Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in MiceReport as inadecuate




Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2015 2015, Article ID 752930, 9 pages -

Research Article

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China

Orthopedics Research Center, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China

Ministry of Education Center for Food Safety of Animal Origin, Dalian University of Technology, Dalian 116620, China

Received 31 October 2014; Revised 19 January 2015; Accepted 4 March 2015

Academic Editor: Teresa M. Coque

Copyright © 2015 Fang Cao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Multidrug-resistant Klebsiella pneumoniae MRKP has steadily grown beyond antibiotic control. However, a bacteriophage is considered to be a potential antibiotic alternative for treating bacterial infections. In this study, a lytic bacteriophage, phage 1513, was isolated using a clinical MRKP isolate KP 1513 as the host and was characterized. It produced a clear plaque with a halo and was classified as Siphoviridae. It had a short latent period of 30 min, a burst size of 264 and could inhibit KP 1513 growth in vitro with a dose-dependent pattern. Intranasal administration of a single dose of 2 × 10

 PFU-mouse 2 h after KP 1513 inoculation was able to protect mice against lethal pneumonia. In a sublethal pneumonia model, phage-treated mice exhibited a lower level of K. pneumoniae burden in the lungs as compared to the untreated control. These mice lost less body weight and exhibited lower levels of inflammatory cytokines in their lungs. Lung lesion conditions were obviously improved by phage therapy. Therefore, phage 1513 has a great effect in vitro and in vivo, which has potential to be used as an alternative to an antibiotic treatment of pneumonia that is caused by the multidrug-resistant K. pneumoniae.





Author: Fang Cao, Xitao Wang, Linhui Wang, Zhen Li, Jian Che, Lili Wang, Xiaoyu Li, Zhenhui Cao, Jiancheng Zhang, Liji Jin, and

Source: https://www.hindawi.com/



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