Total antioxidant capacity – a novel early bio-chemical marker of oxidative stress in HIV infected individualsReport as inadecuate




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Journal of Biomedical Science

, 16:61

First Online: 07 July 2009Received: 02 April 2009Accepted: 07 July 2009DOI: 10.1186-1423-0127-16-61

Cite this article as: Suresh, D., Annam, V., Pratibha, K. et al. J Biomed Sci 2009 16: 61. doi:10.1186-1423-0127-16-61

Abstract

BackgroundOxidative stress induced by the production of reactive oxygen species may play a critical role in the stimulation of HIV replication and the development of immunodeficiency. This study was conducted as there are limited and inconclusive studies on the significance of a novel early marker of oxidative stress which can reflect the total antioxidant capacity in HIV patients,

MethodsTotal antioxidant capacity TAC and lipid peroxidation were evaluated in 50 HIV-1 seropositive patients including HIV-1 symptomatics and asymptomatics. Controls included 50 age and sex matched and apparently healthy HIV-1 seronegative subjects. Serum malondialdehyde MDA, Total antioxidant capacity TAC by ferric reducing antioxidant power assay, vitamin E, vitamin C and superoxide dismutase SOD enzyme activity were estimated among controls and cases. Statistical comparisons and correlations at 5% level of significance were determined.

Results and DiscussionThe mean MDA concentrations were significantly elevated in both HIV-1 asymptomatic CD4+ count > 500 cells-microliter and HIV-1 symptomatic CD4+ count <500 cells-microliter groups Mean ± S.D values were 2.2 +- 0.7 nmol-ml and 2.8 +- 0.8 nmol-ml respectively when compared with the control group Mean ± S.D value was 0.9 +- 0.2 nmol-ml p < 0.01. The mean TAC of HIV- 1 asymptomatic and HIV-1 symptomatic Mean ± S.D values were 754.6 ± 135.6 μmol-L and 676.6 ± 154.1 μmol-L respectively patients were significantly reduced compared with the control group Mean ± S.D value was 1018.7 ± 125.6 μmol-L p < 0.01. Also, there were significantly decreased levels of vitamin E, vitamin C and SOD among HIV-1 seropositive patientscontrols > asymptomatic > symptomatic compared to controls p < 0.01. TAC showed significant negative correlation with MDA among HIV-1 infected patients p < 0.01.

ConclusionOur results clearly show that severe oxidative stress occurs in the HIV-1 seropositive patients in comparison with controls, and increases significantly with the progression of disease, i.e. HIV-1 symptomatics > asymptomatics > controls. TAC can be used as a novel early bio-chemical marker of oxidative stress in HIV-1 infected patients which may result in reduced tissue damage by free radicals and help to monitor and optimize antioxidant therapy in such patients.

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Author: DR Suresh - Vamseedhar Annam - K Pratibha - BV Maruti Prasad

Source: https://link.springer.com/







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