Substance P scavenger enhances antioxidant defenses and prevents prothrombotic effects on the rat lung after acute exposure to oil smokeReport as inadecuate




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Journal of Biomedical Science

, 16:58

First Online: 06 July 2009Received: 28 November 2008Accepted: 06 July 2009DOI: 10.1186-1423-0127-16-58

Cite this article as: Ping-Chia, L., I-Ju, L., Yu-Ching, L. et al. J Biomed Sci 2009 16: 58. doi:10.1186-1423-0127-16-58

Abstract

BackgroundAirborne particulate matter, from cooking oil, smoking, engine exhaust and other sources, is associated with the development of atherosclerosis and myocardial infarction. In order to explore the cellular and molecular events following exposure of rats to lard oil smoke, we measured the generation of reactive oxygen species ROS, substance P, cellular adhesion molecules, and thrombosis in relation to inhibitors of substance P, the NK-1 receptor, and antioxidants.

MethodsRats were exposed to oil smoke for 120 min with or without 20 min pretreatment with lovastatin substance P scavenger, L733060 NK-1 receptor antagonist, vitamin E antioxidant or catechins antioxidant. The levels of substance P and ROS were measured. Histological studies observed ROS damage in the form of HEL adducts. The prothrombotic effects of oil smoke exposure were measured by experimental induction of thrombosis in vivo.

ResultsOil smoke exposure significantly increased substance P levels, ROS levels, ROS damage HEL adduct levels, and the size of experimentally induced thrombi. The pretreatments reduced all of these effects of oil smoke exposure; at many time points the reductions were statistically significant.

ConclusionWe established a connection between oil smoke exposure and thrombosis which involves substance P and its receptor, the NK-1 receptor, and ROS. This study helps establish a mechanistic explanation of how airborne particulate matter can increase the risk of cardiovascular illness.

Electronic supplementary materialThe online version of this article doi:10.1186-1423-0127-16-58 contains supplementary material, which is available to authorized users.

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Author: Li Ping-Chia - Lai I-Ju - Lin Yu-Ching - Chang Li-Ching - Chen Wen-Chung

Source: https://link.springer.com/







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