Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa CellsReport as inadecuate




Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2017 2017, Article ID 9738640, 9 pages - https:-doi.org-10.1155-2017-9738640

Research Article

Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland

Department of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland

Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Obilni trh 11, 602 00 Brno, Czech Republic

Department of Anatomy and Histology, Faculty of Medicine and Health Sciences, University of Zielona Gora, Ul. Zyty 28, 65-046 Zielona Gora, Poland

Institute of Veterinary and Animal Science, Poznan University of Life Sciences, 52 Wojska Polskiego St., 60-628 Poznań, Poland

Correspondence should be addressed to Bartosz Kempisty

Received 17 October 2016; Revised 9 January 2017; Accepted 1 February 2017; Published 27 February 2017

Academic Editor: Takashi Yazawa

Copyright © 2017 Sylwia Ciesiółka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells GCs during folliculogenesis, oogenesis, and oocyte maturation. Although the role of 17 beta-estradiol E2 in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection GJC formation, and GCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin Cx, luteinizing hormone receptor LHR, follicle stimulating hormone receptor FSHR, and aromatase CYP19A1 expression during short-term 168 h primary culture. The cultured GCs were exposed to acute at 96 h of culture and-or prolonged between 0 and 168 h of culture administration of 1.8 and 3.6 μM E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1 mRNA was measured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, and CYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.





Author: Sylwia Ciesiółka, Joanna Budna, Karol Jopek, Artur Bryja, Wiesława Kranc, Sylwia Borys, Michal Jeseta, Adrian Chachuła,

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents