Treatment with TNF-α or bacterial lipopolysaccharide attenuates endocardial endothelial cell-mediated stimulation of cardiac fibroblastsReport as inadecuate




Treatment with TNF-α or bacterial lipopolysaccharide attenuates endocardial endothelial cell-mediated stimulation of cardiac fibroblasts - Download this document for free, or read online. Document in PDF available to download.

Journal of Biomedical Science

, 16:21

First Online: 17 February 2009Received: 16 August 2008Accepted: 17 February 2009DOI: 10.1186-1423-0127-16-21

Cite this article as: Kuruvilla, L. & Kartha, C.C. J Biomed Sci 2009 16: 21. doi:10.1186-1423-0127-16-21

Abstract

BackgroundThe endocardial endothelium that lines the inner cavity of the heart is distinct from the microvascular endothelial cells and modulates cardiac muscle performance in a manner similar to the vascular endothelial modulation of vascular structure and vasomotor tone. Although the modulatory effects of endocardial endothelium EE on cardiomyocytes are firmly established, the regulatory effects of endocardial endothelium on the cardiac interstitium and its cellular components remain ill defined.

Methods and ResultsWe investigated whether the stimulatory effect of EE on cardiac fibroblasts would be altered when EECs are activated by the cytokine tumor necrosis factor-α TNF-α or the endotoxin bacterial lipopolysaccharide LPS. Both TNF-α and LPS were found to independently attenuate the stimulatory effect of EE on cardiac fibroblasts. These agents lowered the synthesis or release of ET-1 and increased the secretion of TGF-β and NO.

ConclusionThe findings of this study using endocardial endothelial cells EECs and neonatal cardiac fibroblasts demonstrate that pro-inflammatory cytokines cause altered secretion of paracrine factors by EECs and inhibit proliferation and lower collagen synthesis in fibroblasts. These changes may influence fibroblast response and extra cellular matrix remodeling in pathological conditions of the heart.

Electronic supplementary materialThe online version of this article doi:10.1186-1423-0127-16-21 contains supplementary material, which is available to authorized users.

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Author: Leena Kuruvilla - Cheranellore Chandrasekharan Kartha

Source: https://link.springer.com/



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