Bioinformatics for the NuGO proof of principle study: analysis of gene expression in muscle of ApoE3*Leiden mice on a high-fat diet using PathVisioReport as inadecuate




Bioinformatics for the NuGO proof of principle study: analysis of gene expression in muscle of ApoE3*Leiden mice on a high-fat diet using PathVisio - Download this document for free, or read online. Document in PDF available to download.

Genes and Nutrition

, Volume 3, Issue 3–4, pp 185–191

First Online: 26 November 2008Received: 08 October 2008Accepted: 12 November 2008DOI: 10.1007-s12263-008-0100-7

Cite this article as: Coort, S.L.M., van Iersel, M.P., van Erk, M. et al. Genes Nutr 2008 3: 185. doi:10.1007-s12263-008-0100-7

Abstract

Insulin resistance is a characteristic of type-2 diabetes and its development is associated with an increased fat consumption. Muscle is one of the tissues that becomes insulin resistant after high fat HF feeding. The aim of the present study is to identify processes involved in the development of HF-induced insulin resistance in muscle of ApOE3*Leiden mice by using microarrays. These mice are known to become insulin resistant on a HF diet. Differential gene expression was measured in muscle using the Affymetrix mouse plus 2.0 array. To get more insight in the processes, affected pathway analysis was performed with a new tool, PathVisio. PathVisio is a pathway editor customized with plug-ins 1 to visualize microarray data on pathways and 2 to perform statistical analysis to select pathways of interest. The present study demonstrated that with pathway analysis, using PathVisio, a large variety of processes can be investigated. The significantly regulated genes in muscle of ApOE3*Leiden mice after 12 weeks of HF feeding were involved in several biological pathways including fatty acid beta oxidation, fatty acid biosynthesis, insulin signaling, oxidative stress and inflammation.

KeywordsPathway analysis Microarray Insulin resistance High-fat diet  Download fulltext PDF



Author: Susan L. M. Coort - Martijn P. van Iersel - Marjan van Erk - Teake Kooistra - Robert Kleemann - Chris T. A. Evelo

Source: https://link.springer.com/







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