Urinary Metabolomics on the Biochemical Profiles in Diet-Induced Hyperlipidemia Rat Using Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight SYNAPT High-Definition Mass SpectrometryReport as inadecuate




Urinary Metabolomics on the Biochemical Profiles in Diet-Induced Hyperlipidemia Rat Using Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight SYNAPT High-Definition Mass Spectrometry - Download this document for free, or read online. Document in PDF available to download.

Journal of Analytical Methods in Chemistry - Volume 2014 2014, Article ID 184162, 9 pages -

Research Article

Department of Physical Education, Northwest University, No. 229 Taibai North Road, Xi’an, Shaanxi 710069, China

Department of Traditional Chinese Medicine, The College of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi’an, Shaanxi 710069, China

National Institutes for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China

Solution Centre, Waters Technologies Shanghai Ltd., No. 1000 Jinhai Road, Shanghai 201203, China

Received 8 January 2014; Accepted 23 January 2014; Published 18 March 2014

Academic Editor: Ying-Yong Zhao

Copyright © 2014 Hua Miao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ultraperformance liquid chromatography coupled with quadrupole time-of-flight synapt high-definition mass spectrometry metabolomics was used to characterize the urinary metabolic profiling of diet-induced hyperlipidaemia in a rat model. Analysis was done by orthogonal partial least squares discriminant analysis, correlation analysis, heat map analysis, and KEGG pathways analysis. Potential biomarkers were chosen by S-plot and were identified by accurate mass, isotopic pattern, and MS-MS fragments information. Significant differences in fatty acid, amino acid, nucleoside, and bile acid were observed, indicating the perturbations of fatty acid, amino acid, nucleoside, and bile acid metabolisms in diet-induced hyperlipidaemia rats. This study provides further insight into the metabolic profiling across a wide range of biochemical pathways in response to diet-induced hyperlipidaemia.





Author: Hua Miao, Hua Chen, Xu Zhang, Lu Yin, Dan-Qian Chen, Xian-Long Cheng, Xu Bai, and Feng Wei

Source: https://www.hindawi.com/



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