Aminated β-Cyclodextrin-Modified-Carboxylated Magnetic Cobalt-Nanocellulose Composite for Tumor-Targeted Gene DeliveryReport as inadecuate




Aminated β-Cyclodextrin-Modified-Carboxylated Magnetic Cobalt-Nanocellulose Composite for Tumor-Targeted Gene Delivery - Download this document for free, or read online. Document in PDF available to download.

Journal of Applied Chemistry - Volume 2014 2014, Article ID 184153, 10 pages -

Research ArticleDepartment of Chemistry, University of Kerala, Kariavattom, Trivandrum 695 581, India

Received 18 August 2014; Accepted 6 December 2014; Published 31 December 2014

Academic Editor: Rassoul Dinarvand

Copyright © 2014 Thayyath Sreenivasan Anirudhan and Sylaja Raveendran Rejeena. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Gene therapy is a new kind of medicine, which uses genes as drugs in order to treat life threatening diseases. In the present work, a nonviral vector, aminated β-cyclodextrin-modified-carboxylated magnetic cobalt-nanocellulose composite ACDC-Co-NCC, was synthesized for efficient transfection of genes into tumour cells. The synthesized ACDC-Co-NCC was characterized by means of FTIR, XRD, SEM, and ESR techniques. DNA condensing ability of ACDC-Co-NCC was found to be increased with increase in amount of ACDC-Co-NCC and 84.9% of DNA 1.0 μg-mL inclusion was observed with 6.0 μg-mL of ACDC-Co-NCC. The cytotoxicity of ACDC-Co-NCC was observed to be minimal, even at higher concentration, with respect to the model transfecting agent, polyethyleneimine PEI. 88.2% of the gene was transfected at high dose of DNA, as indicated by the highest luciferase expression. These results indicated that ACDC-Co-NCC might be a promising candidate for gene delivery with the characteristics of good biocompatibility, potential biodegradability, minimal cytotoxicity, and relatively high gene transfection efficiency.





Author: Thayyath Sreenivasan Anirudhan and Sylaja Raveendran Rejeena

Source: https://www.hindawi.com/



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