Dexamethasone inhibits BAFF expression in fibroblast-like synoviocytes from patients with rheumatoid arthritisReport as inadecuate




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Fibroblast-like synoviocytes FLS play a major role in the pathogenesis of rheumatoid arthritis RA. FLS isolated from patientswith RA FLS-RA express B cell-activating factor belonging to the TNF family BAFF, a cytokine that has been associated withthe onset and progression of RA. Glucocorticoids are powerful anti-inflammatory drugs used in the treatment of RA. In the presentstudy, we examined the effect of dexamethasone Dex on constitutive and TNF-a- and IFN-c-induced BAFF expression in FLS-RA.BAFF mRNA expression and soluble BAFF were determined by RT-PCR and ELISA, respectively. The results showed that constitutiveBAFF mRNA expression was inhibited by Dex in a dose- and time-dependent manner. Also, Dex inhibited the secretion of BAFF in atime-dependent manner reaching 76% of inhibition 72 h after treatment. Moreover, Dex suppressed both mRNA and protein BAFFexpression induced by TNF-a but had no effect on IFN-c-induced BAFF expression. In comparison with B cells cultured alone, B cellsco-cultured with FLS-RA exhibited a higher survival, which was inhibited when FLS-RA were pretreated with Dex. However, theenhanced B cell survival was reestablished by the addition of rhBAFF. Therefore, Dex is a potent inhibitor of constitutive and TNFa-induced BAFF expression in FLS-RA.



Author: Reyes, Lilian I.; - León, Francisca; - González, Patricia; - Rozas, María F.; - Labarca, Cristián; - Segovia, Alejandra; - Na

Source: http://repositorio.uchile.cl/



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