Increased human defensine levels hint at an inflammatory etiology of bisphosphonate-associated osteonecrosis of the jaw: An immunohistological studyReport as inadecuate




Increased human defensine levels hint at an inflammatory etiology of bisphosphonate-associated osteonecrosis of the jaw: An immunohistological study - Download this document for free, or read online. Document in PDF available to download.

Journal of Translational Medicine

, 9:135

First Online: 15 August 2011Received: 09 March 2011Accepted: 15 August 2011DOI: 10.1186-1479-5876-9-135

Cite this article as: Stockmann, P., Wehrhan, F., Schwarz-Furlan, S. et al. J Transl Med 2011 9: 135. doi:10.1186-1479-5876-9-135

Abstract

BackgroundHuman β-defensins hBD are antimicrobial peptides that are an integral part of bone innate immunity. Recently, it could be shown that expression of hBD-1 -2 and -3 were upregulated in cases of osteomyelitis of the jaws. In order to gain insight into the possible impairment of hBD metabolism in bisphosphonate-associated osteonecrosis of the jaws BONJ, the present exploratory study was designed so as to determine the qualitative and quantitative expression of afore mentioned hBDs in BONJ and infected osteoradionecrosis ORN, both of which represent inflammatory bone diseases.

MethodsBone samples were collected from patients with BONJ n = 20 and ORN n = 20. Non-infected healthy bone samples n = 20 were included as controls. Immunohistological staining in an autostainer was carried out by the Strept-ABC-method against hBD-1,-2,-3. Specific positive vs. negative cell reaction of osteocytes labeling index near the border of bony resection was determined and counted for quantitative analysis. Number of vital osteocytes vs. empty osteocytes lacunae was compared between groups.

ResultshBD-1,-2 and -3 could be detected in BONJ as well as ORN and healthy bone samples. Immunoreactivity against hBD-2 and -3 was significantly higher in BONJ than in ORN and healthy jaw bone samples. Number of empty osteocyte lacunae was significantly higher in ORN compared with BONJ P = 0.001.

ConclusionUnder the condition of BONJ an increased expression of hBD-1,-2,-3 is detectable, similarly to the recently described upregulation of defensins in chronically infected jaw bones. It remains still unclear how these findings may relate to the pathoetiology of these diseases and whether this is contributing to the development of BONJ and ORN or simply an after effect of the disease.

Keywordsantimicrobial peptide bisphosphonate-associated osteonecrosis osteoradionecrosis human beta defensins innate immunity Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-9-135 contains supplementary material, which is available to authorized users.

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