Long range molecular dynamics study of regulation of eukaryotic glucosamine-6-phosphate synthase activity by UDP-GlcNAcReport as inadecuate




Long range molecular dynamics study of regulation of eukaryotic glucosamine-6-phosphate synthase activity by UDP-GlcNAc - Download this document for free, or read online. Document in PDF available to download.

Journal of Molecular Modeling

, Volume 17, Issue 12, pp 3103–3115

First Online: 02 March 2011Received: 14 August 2010Accepted: 28 January 2011DOI: 10.1007-s00894-011-1003-x

Cite this article as: Miszkiel, A., Wojciechowski, M. & Milewski, S. J Mol Model 2011 17: 3103. doi:10.1007-s00894-011-1003-x

Abstract

Glucosamine-6-phosphate GlcN-6-P synthase catalyses the first and practically irreversible step in hexosamine metabolism. The final product of this pathway, uridine 5’ diphospho N-acetyl-D-glucosamine UDP-GlcNAc, is an essential substrate for assembly of bacterial and fungal cell walls. Moreover, the enzyme is involved in phenomenon of hexosamine induced insulin resistance in type II diabetes, which makes it a potential target for antifungal, antibacterial and antidiabetic therapy. The crystal structure of the isomerase domain of GlcN-6-P synthase from human pathogenic fungus Candida albicans, in complex with UDP-GlcNAc has been solved recently but it has not revealed the molecular mechanism of inhibition taking place under UDP-GlcNAc influence, the unique feature of the eukaryotic enzyme. UDP-GlcNAc is a physiological inhibitor of GlcN-6-P synthase, binding about 1 nm away from the active site of the enzyme. In the present work, comparative molecular dynamics simulations of the free and UDP-GlcNAc-bounded structures of GlcN-6-P synthase have been performed. The aim was to complete static X-ray structural data and detect possible changes in the dynamics of the two structures. Results of the simulation studies demonstrated higher mobility of the free structure when compared to the liganded one. Several amino acid residues were identified, flexibility of which is strongly affected upon UDP-GlcNAc binding. Importantly, the most fixed residues are those related to the inhibitor binding process and to the catalytic reaction. The obtained results constitute an important step toward understanding of mechanism of GlcN-6-P synthase inhibition by UDP-GlcNAc molecule.

KeywordsCosine content Glucosamine-6-phosphate synthase Molecular dynamics Principal component analysis UDP-GlcNAc  Download fulltext PDF



Author: Aleksandra Miszkiel - Marek Wojciechowski - Sławomir Milewski

Source: https://link.springer.com/







Related documents