Development and characterization of highly polymorphic long TC repeat microsatellite markers for genetic analysis of peanutReport as inadecuate




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BMC Research Notes

, 5:86

First Online: 03 February 2012Received: 25 October 2011Accepted: 03 February 2012DOI: 10.1186-1756-0500-5-86

Cite this article as: Macedo, S.E., Moretzsohn, M.C., M Leal-Bertioli, S.C. et al. BMC Res Notes 2012 5: 86. doi:10.1186-1756-0500-5-86

Abstract

BackgroundPeanut Arachis hypogaea L. is a crop of economic and social importance, mainly in tropical areas, and developing countries. Its molecular breeding has been hindered by a shortage of polymorphic genetic markers due to a very narrow genetic base. Microsatellites SSRs are markers of choice in peanut because they are co-dominant, highly transferrable between species and easily applicable in the allotetraploid genome. In spite of substantial effort over the last few years by a number of research groups, the number of SSRs that are polymorphic for A. hypogaea is still limiting for routine application, creating the demand for the discovery of more markers polymorphic within cultivated germplasm.

FindingsA plasmid genomic library enriched for TC-AG repeats was constructed and 1401 clones sequenced. From the sequences obtained 146 primer pairs flanking mostly TC microsatellites were developed. The average number of repeat motifs amplified was 23. These 146 markers were characterized on 22 genotypes of cultivated peanut. In total 78 of the markers were polymorphic within cultivated germplasm. Most of those 78 markers were highly informative with an average of 5.4 alleles per locus being amplified. Average gene diversity index GD was 0.6, and 66 markers showed a GD of more than 0.5. Genetic relationship analysis was performed and corroborated the current taxonomical classification of A. hypogaea subspecies and varieties.

ConclusionsThe microsatellite markers described here are a useful resource for genetics and genomics in Arachis. In particular, the 66 markers that are highly polymorphic in cultivated peanut are a significant step towards routine genetic mapping and marker-assisted selection for the crop.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-5-86 contains supplementary material, which is available to authorized users.

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Author: Selma E Macedo - Márcio C Moretzsohn - Soraya C M Leal-Bertioli - Dione MT Alves - Ediene G Gouvea - Vânia CR Azeved

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