Expression of the phosphorylated MEK5 protein is associated with TNM staging of colorectal cancerReport as inadecuate




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BMC Cancer

, 12:127

First Online: 30 March 2012Received: 05 December 2011Accepted: 30 March 2012DOI: 10.1186-1471-2407-12-127

Cite this article as: Hu, B., Ren, D., Su, D. et al. BMC Cancer 2012 12: 127. doi:10.1186-1471-2407-12-127

Abstract

BackgroundActivation of MEK5 in many cancers is associated with carcinogenesis through aberrant cell proliferation. In this study, we determined the level of phosphorylated MEK5 pMEK5 expression in human colorectal cancer CRC tissues and correlated it with clinicopathologic data.

MethodspMEK5 expression was examined by immunohistochemistry in a tissue microarray TMA containing 335 clinicopathologic characterized CRC cases and 80 cases of nontumor colorectal tissues. pMEK5 expression of 19 cases of primary CRC lesions and paired with normal mucosa was examined by Western blotting. The relationship between pMEK5 expression in CRC and clinicopathologic parameters, and the association of pMEK5 expression with CRC survival were analyzed respectively.

ResultspMEK5 expression was significantly higher in CRC tissues 185 out of 335, 55.2% than in normal tissues 6 out of 80, 7.5%; P < 0.001. Western blotting demonstrated that pMEK5 expression was upregulated in 12 of 19 CRC tissues 62.1% compared to the corresponding adjacent nontumor colorectal tissues. Overexpression of pMEK5 in CRC tissues was significantly correlated to the depth of invasion P = 0.001, lymph node metastasis P < 0.001, distant metastasis P < 0.001 and high preoperative CEA level P < 0.001. Consistently, the pMEK5 level in CRC tissues was increased following stage progression of the disease P < 0.001. Analysis of the survival curves showed a significantly worse 5-year disease-free P = 0.002 and 5-year overall survival rate P < 0.001 for patients whose tumors overexpressed pMEK5. However, in multivariate analysis, pMEK5 was not an independent prognostic factor for CRC DFS: P = 0.139; OS: P = 0.071.

ConclusionspMEK5 expression is correlated with the staging of CRC and its expression might be helpful to the TNM staging system of CRC.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-12-127 contains supplementary material, which is available to authorized users.

Bang Hu, Donglin Ren contributed equally to this work.

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Author: Bang Hu - Donglin Ren - Dan Su - Hongcheng Lin - Zhenyu Xian - Xingyang Wan - Junxiao Zhang - Xinhui Fu - Li Jiang - Dech

Source: https://link.springer.com/







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