Does Implant Coating With Antibacterial-Loaded Hydrogel Reduce Bacterial Colonization and Biofilm Formation in VitroReport as inadecuate




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Clinical Orthopaedics and Related Research®

, Volume 472, Issue 11, pp 3311–3323

First Online: 13 March 2014DOI: 10.1007-s11999-014-3558-1

Cite this article as: Drago, L., Boot, W., Dimas, K. et al. Clin Orthop Relat Res 2014 472: 3311. doi:10.1007-s11999-014-3558-1

Abstract

BackgroundImplant-related infections represent one of the most severe complications in orthopaedics. A fast-resorbable, antibacterial-loaded hydrogel may reduce or prevent bacterial colonization and biofilm formation of implanted biomaterials.

Questions-purposesWe asked: 1 Is a fast-resorbable hydrogel able to deliver antibacterial compounds in vitro? 2 Can a hydrogel alone or antibacterial-loaded coating on implants reduce bacterial colonization? And 3 is intraoperative coating feasible and resistant to press-fit implant insertion?

MethodsWe tested the ability of Disposable Antibacterial Coating DAC hydrogel Novagenit Srl, Mezzolombardo, Italy to deliver antibacterial agents using spectrophotometry and a microbiologic assay. Antibacterial and antibiofilm activity were determined by broth microdilution and a crystal violet assay, respectively. Coating resistance to press-fit insertion was tested in rabbit tibias and human femurs.

ResultsComplete release of all tested antibacterial compounds was observed in less than 96 hours. Bactericidal and antibiofilm effect of DAC hydrogel in combination with various antibacterials was shown in vitro. Approximately 80% of the hydrogel coating was retrieved on the implant after press-fit insertion.

ConclusionsImplant coating with an antibacterial-loaded hydrogel reduces bacterial colonization and biofilm formation in vitro.

Clinical Relevance A fast-resorbable, antibacterial-loaded hydrogel coating may help prevent implant-related infections in orthopaedics. However, further validation in animal models and properly controlled human studies is required.

Each author certifies that he or she, or a member of his o her immediate family, has no commercial associations eg, consultancies, stock ownership, equity interest, patent-licensing arrangements, etc that might pose a conflict of interest in connection with the submitted article. The present research was conducted under the multicenter collaborative project -Implant Disposable Antibacterial Coating IDAC: A Novel Approach to Implant-Related Infections in Orthopaedics and Trauma Surgery,- funded by the European Commission, within the Seventh Framework Programme on Research Technological Development and Demonstration under Grant 277988.

All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research editors and board members are on file with the publication and can be viewed on request.

Each author certifies that his or her institution approved the animal protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.

The study was performed at the Central Laboratory Animal Research Facility of the University Medical Center Utrecht, The Netherlands.

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Author: Lorenzo Drago - Willemijn Boot - Kostantinos Dimas - Kostantinos Malizos - Gertrud M. Hänsch - Jos Stuyck - Debby Gawlitt

Source: https://link.springer.com/







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