Tumor suppressor genes are frequently methylated in lymph node metastases of breast cancersReport as inadecuate




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BMC Cancer

, 10:378

Genetics, genomics and epigenetics

Abstract

IntroductionMetastasis represents a major adverse step in the progression of breast carcinoma. Lymph node invasion is the most relevant prognostic factor; however little is known on the molecular events associated with lymph node metastasis process. This study is to investigate the status and role of methylation in lymph node metastatic tumors.

Materials and methodsBisulfite pyrosequencing is used to screen 6 putative tumor suppressor genes HIN-1, RASSF1A, RIL, CDH13, RARβ2 and E-cadherin in 38 pairs of primary breast tumors and lymph node metastases.

ResultsWe found that HIN-1, CDH13, RIL, RASSF1A and RARβ2 were frequently methylated both in primary and metastatic tissues range: 55.3%~89.5%. E-cadherin was not frequently methylated in either setting range: 18.4%~23.7%. The methylation status of HIN-1, CDH13, RIL, and RARβ2 in lymph nodes metastasis were correlated with that in primary tumors. The Pearson correlation values ranged from 0.624 to 0.472 p values < 0.01 to 0.001. Interestingly, we observed an association between HIN-1 methylation and hormone status in metastatic lymph nodes. Hypermethylation of HIN-1 in metastasis lymph nodes was significantly associated with expression of ER odds ratio, 1.070; P = 0.024 and with PR odds ratio, 1.046; P = 0.026.

ConclusionsThis study suggests that hypermethylation of tumor suppressor genes is extended from primary to metastatic tumors during tumor progression.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-10-378 contains supplementary material, which is available to authorized users.

Weiwei Feng, Rosaria Orlandi contributed equally to this work.

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Author: Weiwei Feng - Rosaria Orlandi - Naiqing Zhao - Maria Luisa Carcangiu - Elda Tagliabue - Jia Xu - Robert C BastJr - Yinhua

Source: https://link.springer.com/







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