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European Journal of Medical Research

, 20:22

First Online: 12 March 2015Received: 06 July 2014Accepted: 20 February 2015DOI: 10.1186-s40001-015-0114-8

Cite this article as: Liu, WJ., Zhang, XM., Wang, N. et al. Eur J Med Res 2015 20: 22. doi:10.1186-s40001-015-0114-8

Abstract

BackgroundSilent information regulator 2 related enzyme 1 SIRT1 is one of the key factors in the mechanism of calorie restriction CR extending lifespan of animals. The aim of the study is to investigate if CR prolongs ovarian lifespan in mice through activating SIRT1 signaling.

MethodsIn the present study, 21 female C57BL-6 mice were divided into three groups: the control n = 7, CR n = 7, and SRT1720 n = 7 groups. After the 26-week treatment, the number of ovarian follicles at each stage was counted, and Western blot was performed.

ResultsThe number of surviving follicles in ovaries of the SRT1720 group was less than that of the CR group but more than that of the normal control NC group. The number of atretic follicles in the ovaries of the SRT1720 group was similar to that of the CR group but less than that of the NC group. The number of primordial follicles in the ovaries of the SRT1720 group was less than that of the CR group but more than that of the NC group. The numbers of primary follicles, secondary follicles, antral follicles, and corpora lutea in the SRT1720 group were similar to those in the CR group. Western blot analysis showed that the expression of SIRT1, SIRT6, FOXO3a, and NRF1 proteins was upregulated, and p53 was downregulated in both the CR group and the SRT1720 group compared to the control group.

ConclusionsOur results indicate that CR inhibits the activation of primordial follicles and development of follicles at different stages, thus preserving the reserve of follicle pool at least partly through activating SIRT1 signaling.

KeywordsCalorie restriction Ovarian development SIRT1 signaling SIRT1 activator Mice AbbreviationsCRcalorie restriction

FOXO3aforkhead box group O

HEhematoxylin and eosin

NCnormal control

NRF1nuclear respiratory factor 1

PAGEpolyacryl amide gel electrophoresis

PBSphosphate buffer solution

PGpre-granular cell

POFpremature ovarian failure

SEMstandard error of mean

SDSsodium dodecyl sulfate

SIRT1silent information regulator 2 related enzyme 1

SIRT6silent information regulator 2 related enzyme 6

SPSSStatistical Package for the Social Sciences

TBSTtris-buffered-saline with tween

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Author: Wei-Juan Liu - Xing-Mei Zhang - Na Wang - Xiao-Ling Zhou - Yu-Cai Fu - Li-Li Luo

Source: https://link.springer.com/







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