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BMC Cardiovascular Disorders

, 10:19

First Online: 21 April 2010Received: 28 August 2009Accepted: 21 April 2010DOI: 10.1186-1471-2261-10-19

Cite this article as: Barascuk, N., Skjøt-Arkil, H., Register, T.C. et al. BMC Cardiovasc Disord 2010 10: 19. doi:10.1186-1471-2261-10-19

Abstract

BackgroundProteolytic degradation of Type I Collagen by proteases may play an important role in remodeling of atherosclerotic plaques, contributing to increased risk of plaque rupture.

The aim of the current study was to investigate whether human macrophage foam cells degrade the extracellular matrix ECM of atherosclerotic plaques by cathepsin K mediated processes.

MethodsWe 1 cultured human macrophages on ECM and measured cathepsin K generated fragments of type I collagen C-terminal fragments of Type I collagen CTX-I 2 investigated the presence of CTX-I in human coronary arteries and 3 finally investigated the clinical potential by measuring circulating CTX-I in women with and without radiographic evidence of aortic calcified atherosclerosis.

ResultsImmune-histochemistry of early and advanced lesions of coronary arteries demonstrated co-localization of Cathepsin-K and CTX-I in areas of intimal hyperplasia and in shoulder regions of advanced plaques. Treatment of human monocytes with M-CSF or M-CSF+LDL generated macrophages and foam cells producing CTX-I when cultured on type I collagen enriched matrix. Circulating levels of CTX-I were not significantly different in women with aortic calcifications compared to those without.

ConclusionsHuman macrophage foam cells degrade the atherosclerotic plaques though cathepsin K mediated processes, resulting in increase in levels of CTX-I. Serum CTX-I was not elevated in women with aortic calcification, likely due to the contribution of CTX-I from osteoclastic bone resorption which involves Cathepsin-K. The human macrophage model system may be used to identify important pathway leading to excessive proteolytic plaque remodeling and plaque rupture.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2261-10-19 contains supplementary material, which is available to authorized users.

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Author: Natasha Barascuk - Helene Skjøt-Arkil - Thomas C Register - Lise Larsen - Inger Byrjalsen - Claus Christiansen - Morten A

Source: https://link.springer.com/







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