A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine maxReport as inadecuate




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BMC Research Notes

, 6:255

First Online: 06 July 2013Received: 19 December 2012Accepted: 03 July 2013DOI: 10.1186-1756-0500-6-255

Cite this article as: Kandoth, P.K., Heinz, R., Yeckel, G. et al. BMC Res Notes 2013 6: 255. doi:10.1186-1756-0500-6-255

Abstract

BackgroundBean pod mottle virus BPMV based virus-induced gene silencing VIGS vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

FindingsHere we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode SCN. A detailed SCN screening pipeline is described.

ConclusionsThe VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.

KeywordsHeterodera glycines Resistance SCN Soybean Cyst nematode Resistance Virus-induced gene silencing VIGS Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-6-255 contains supplementary material, which is available to authorized users.

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Author: Pramod K Kandoth - Robert Heinz - Greg Yeckel - Nathan W Gross - Parijat S Juvale - John Hill - Steven A Whitham - Thom

Source: https://link.springer.com/







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