Occurrence of gustducin-immunoreactive cells in von Ebner’s glands of guinea pigsReport as inadecuate




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Histochemistry and Cell Biology

, Volume 140, Issue 5, pp 567–574

First Online: 19 April 2013Accepted: 20 February 2013DOI: 10.1007-s00418-013-1094-9

Cite this article as: Ibira, Y., Yokosuka, H., Haga-Tsujimura, M. et al. Histochem Cell Biol 2013 140: 567. doi:10.1007-s00418-013-1094-9

Abstract

An immunohistochemical examination of guinea-pig taste buds in vallate papillae revealed gustducin-immunoreactive cells in the area of von Ebner’s glands, minor salivary glands. Since there have been no reports describing those cells in these locations for other species, we investigated these glands in order both to localize the cells and compare their immunoreactive characteristics with corresponding cells in the vallate taste buds. The gustducin-immunoreactive cells coincided with cells containing no secretory granules in the end portion of the glands, which was supported by the electron-microscopic immunocytochemistry. Double immunofluorescence microscopy confirmed these cells to be entirely immunopositive to type III inositol 1,4,5-triphosphate receptor IP3R-3, phospholipase Cβ2 PLCβ2, and villin and also partly immunopositive to neuron-specific enolase NSE and calbindin D-28K. The gustducin-immunoreactive cells in the vallate taste buds exhibited completely the same immunoreactivities for these five molecules. Accordingly, the present results give credence to a consideration that the gustducin-immunnoreactive cells in both locations are identical in functions e.g., chemo-reception.

KeywordsGuinea pig von Ebner’s glands Taste bud Gustducin-immunoreactive cell Immunohistochemistry  Download fulltext PDF



Author: Yuki Ibira - Hiroyuki Yokosuka - Maiko Haga-Tsujimura - Sumio Yoshie

Source: https://link.springer.com/







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