Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancerReport as inadecuate




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Journal of Translational Medicine

, 11:277

Disease Biomarkers

Abstract

BackgroundPaxillin PXN has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear.

MethodsThe expression of PXN was determined in paired gastric cancer tissues and adjacent normal tissues by Western blotting and real-time PCR. Immunohistochemistry was performed to detect the expression of PXN in 239 gastric cancer patients. Statistical analysis was applied to investigate the correlation between PXN expression and clinicopathological characteristics and prognosis in patients. Additionally, the effects of PXN on gastric cancer cell proliferation and migration were also evaluated.

ResultsPXN was up-regulated in gastric cancer tissues and cell lines as compared with adjacent normal tissues and normal gastric epithelial cell line GES-1. Overexpression of PXN was correlated with distant metastasis P = 0.001 and advanced tumor stage P = 0.021 in gastric cancer patients. Patients with high PXN expression tended to have poor prognosis compared with patients with low PXN expression P < 0.001. Multivariate analysis demonstrated that PXN expression was an independent prognostic factor P = 0.020. Moreover, ectopic expression of PXN promotes cell proliferation and migration in AGS cells whereas knockdown of PXN inhibits cell proliferation and migration in SGC7901 cells.

ConclusionsPXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer.

KeywordsGastric cancer Paxillin Tumor progression Prognosis AbbreviationsPXNPaxillin

TNMTumor, lymph node, distant metastasis

IHCImmunohistochemistry

MTT3-4, 5-dimethylthiazole-2-yl-2, 5-biphenyl tetrazolium bromide

siRNASmall interfering RNA.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-11-277 contains supplementary material, which is available to authorized users.

Dong-liang Chen, Zhi-qiang Wang contributed equally to this work.

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Author: Dong-liang Chen - Zhi-qiang Wang - Chao Ren - Zhao-lei Zeng - De-shen Wang - Hui-yan Luo - Feng Wang - Miao-zhen Qiu - Lon

Source: https://link.springer.com/







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