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Transcriptomic profiling, Assisted reproductive technology, Early porcine embryos

ZHOU, Chi

Supervisor and department: Dyck, Michael Agriculture, Food, and Nutritional Science, University of Alberta

Examining committee member and department: Guan, Leluo Agriculture, Food, and Nutritional Science, University of Alberta Stothard, Paul Agriculture, Food, and Nutritional Science, University of Alberta Klein, Claudia Faculty of Veterinsry Medicine, University of Calgary Dixon, Walter Agriculture, Food, and Nutritional Science, University of Alberta

Department: Department of Agricultural, Food, and Nutritional Science

Specialization: Animal Science

Date accepted: 2013-12-19T13:49:34Z

Graduation date: 2014-06

Degree: Doctor of Philosophy

Degree level: Doctoral

Abstract: There are strong interests in the factors that affect the efficient production of viable porcine embryos using either in vivo or in vitro production methods based on assisted reproductive technologies ART. During the pre-implantation period of development, the porcine embryo exhibits dramatic changes and many key events of embryonic development take place. In this research, a series of studies were carried out in order to identify the critical regulators during the pre-implantation period of porcine embryonic development, and to identify the gene networks that are responsible for the impaired development of embryos produced after different ART manipulations. The detailed transcriptome profile of in vivo-derived -normal- pre-implantation porcine embryos has been characterized by transcriptomic profiling analysis of porcine oocytes and embryos representing nine different developmental stages from GV stage oocytes to day 11 embryos. Results from this research also suggest that the molecular events associated with embryonic genomic activation EGA in porcine pig embryos are probably initiated at, or before, the 4-cell stage. The embryo-activated genes -take-over- the majority of the mRNA profile from 8-cell stage onward, and the second wave of EGA probably peaks around the early blastocyst stage. Further comparative transcriptomic analysis between the in vivo hatched blastocysts HB and HB produced after in vitro ART manipulations parthenogenetic activation PA and somatic cell chromatin transfer CT revealed 1492 and 103 genes that differentially expressed of in PA and CT HB, respectively, in comparison with in vivo HB. Several significantly altered critical gene networks and pathways were identified in the PA- and CT-derived HB. In addition, apoptotic process was predicted to be activated in both PA and CT HB, and the activation of this apoptotic process is likely to be greater in PA HB. Finally, the effect of porcine luteinizing hormone-induced ovulation on the transcriptome of early porcine embryos was also investigated. Overall, result from this research provided useful information for the understanding of the molecular mechanism underlying early porcine embryonic development, and identified several critical genes - gene networks that are likely to contribute to the deficiencies of porcine embryos produced after different ART manipulations.

Language: English

DOI: doi:10.7939-R3319SB11

Rights: Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.





Author: ZHOU, Chi

Source: https://era.library.ualberta.ca/


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University of Alberta Comparative gene expression profiling of early porcine embryos derived from different in vivo and in vitro sources by Chi Zhou A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Animal Science Department of Agricultural, Food and Nutritional Science © Chi Zhou Spring 2014 Edmonton, Alberta Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only.
Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the authors prior written permission. Abstract There are strong interests in the factors that affect the efficient production of viable porcine embryos using either in vivo or in vitro production methods based on assisted reproductive technologies (ART).
During the pre-implantation period of development, the porcine embryo exhibits dramatic changes and many key events of embryonic development take place.
In this research, a series of studies were carried out in order to identify the critical regulators during the pre-implantation period of porcine embryonic development, and to identify the gene networks that are responsible for the impaired development of embryos produced after different ART manipulations.
The detailed transcriptome profile of in vivo-derived ―normal‖ pre-implantation porcine embryos has been characterized by transcriptomic profiling analysis of porcine oocytes and embryos representing nine dif...





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