Distribution of keratins, vimentin, and actin in the testis of two South American camelids: Vicuna Vicugna vicugna and Llama Lama glama. An immunohistochemical studyReport as inadecuate




Distribution of keratins, vimentin, and actin in the testis of two South American camelids: Vicuna Vicugna vicugna and Llama Lama glama. An immunohistochemical study - Download this document for free, or read online. Document in PDF available to download.

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The purpose of the present study was to investigate the pattern of distribution of cytokeratins, vimentin and muscular actin in the testis of vicuna Vicugna vicugna and llama Lama glama two species of camelids native of the Andean high plateau of South America. Testicular biopsies of four vicunas and five llamas were used. Animals were healthy breeders.The tissues were processed by standard immunohistochemistry try with antipancytokeratin AE1-AE3, antikeratin 18 K 18, CAM 5.2 antikeratin 5, 18, and 19, antivimentin, and smooth-muscle-specific antiactin antibodies to track the cytoskeletal pattern of testicular cells. Using AE1-AE3 antibody the immunostaining was found in the epithelial Lining of tubuli recti and rete testis, The reaction was relatively stronger in the apical cytoplasm of epithelial cells. The testicular cells of the two species showed no reaction to K 18 and CAM 5.2 antibodies. Antivimentin antibody stained the basal cytoplasm of the Sertoli cells, the Leydig cells, and the epithelial lining of tubuli recti and rete testis. In the last; two structures the immunostain was relatively more intense in the basal cytoplasm of epithelial cells. Antiactin antibody stained the peritubular cells and the muscle cells of the lamina propria of tubuli recti and rete testis. The presence in these species of only some keratins found in man, its coexpression with vimentin in epithelial lining of tubuli recti and rete testis and the peritubule organization, so different from other ungulates may reflect a differential adaptation of the cytoskeleton to particular reproductive strategies.Nota general

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Author: Rodríguez, Angel; - Rojas, Mariana A.; - Bustos Obregón, Eduardo; - Urquieta, Bessie; - Regadera, Javier; -

Source: http://repositorio.uchile.cl/



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