Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat liversReport as inadecuate




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European Journal of Medical Research

, 20:61

First Online: 22 July 2015Received: 25 April 2015Accepted: 30 June 2015DOI: 10.1186-s40001-015-0152-2

Cite this article as: Wang, L., Zhao, B., Chen, Y. et al. Eur J Med Res 2015 20: 61. doi:10.1186-s40001-015-0152-2

Abstract

BackgroundHeme oxygenase-1 HO-1 protects cells by anti-oxidation, maintaining normal microcirculation and anti-inflammatory under stress. This study investigated the effects of biliary tract external drainage BTED on the expression levels of HO-1 in rat livers.

MethodsBiliary tract external drainage was performed by inserting a cannula into the bile duct. Sixty Sprague–Dawley rats were randomized to the following groups: sham 1 h group; BTED 1 h group; bile duct ligation BDL 1 h group; sham 6 h group and BTED 6 h group. The expression levels of HO-1 mRNA were analyzed using real-time RT-PCR. The expression levels of HO-1 were analyzed using immunohistochemistry.

ResultsThe expression levels of HO-1 mRNA in the liver of the BTED group increased significantly compared with the sham group 1 and 6 h after surgery p < 0.05.The expression levels of HO-1 in the BTED group increased significantly compared with the sham group 1 and 6 h after surgery. The expression levels of HO-1 mRNA in the liver in the BDL group decreased significantly compared with the sham group 1 h after surgery p < 0.05.The expression levels of HO-1 in the BDL group decreased significantly compared with the sham group at this time.

ConclusionBiliary tract external drainages increase the expression levels of HO-1 in the liver.

KeywordsBiliary tract external drainage Heme oxygenase-1  Download fulltext PDF



Author: Lu Wang - Bing Zhao - Ying Chen - Li Ma - Er-Zhen Chen - En-Qiang Mao

Source: https://link.springer.com/







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