Matrix gla protein MGP: an overexpressed and migration-promoting mesenchymal component in glioblastomaReport as inadecuate




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BMC Cancer

, 9:302

First Online: 27 August 2009Received: 16 December 2008Accepted: 27 August 2009DOI: 10.1186-1471-2407-9-302

Cite this article as: Mertsch, S., Schurgers, L.J., Weber, K. et al. BMC Cancer 2009 9: 302. doi:10.1186-1471-2407-9-302

Abstract

BackgroundRecent studies have demonstrated that a molecular subtype of glioblastoma is characterized by overexpression of extracellular matrix ECM-mesenchymal components and shorter survival. Specifically, gene expression profiling studies revealed that matrix gla protein MGP, whose function has traditionally been linked to inhibition of calcification of arteries and cartilage, is overexpressed in glioblastomas and associated with worse outcome.

MethodsIn order to analyze the role of MGP in glioblastomas, we performed expression, migration and proliferation studies.

ResultsReal-time PCR and ELISA assays confirmed overexpression of MGP in glioblastoma biopsy specimens and cell lines at mRNA and protein levels as compared to normal brain tissue. Immunohistochemistry verified positivity of glial tumor cells for MGP. RNAi-mediated knockdown of MGP in three glioma cell lines U343MG, U373MG, H4 led to marked reduction of migration, as demonstrated by wound healing and transwell assays, while no effect on proliferation was seen.

ConclusionOur data suggest that upregulation of MGP and possibly other ECM-related components as well results in unfavorable prognosis via increased migration.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-9-302 contains supplementary material, which is available to authorized users.

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Author: Sonja Mertsch - Leon J Schurgers - Kathrin Weber - Werner Paulus - Volker Senner

Source: https://link.springer.com/







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