14-3-3β protein expression in eosinophilic meningitis caused by Angiostrongylus cantonensis infectionReport as inadecuate




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BMC Research Notes

, 7:97

First Online: 20 February 2014Received: 22 March 2013Accepted: 11 February 2014DOI: 10.1186-1756-0500-7-97

Cite this article as: Tsai, HC., Huang, YL., Chen, YS. et al. BMC Res Notes 2014 7: 97. doi:10.1186-1756-0500-7-97

Abstract

BackgroundAngiostrongylus cantonensis is a parasite endemic in the Southeast Asian and Pacific regions. Humans are incidentally infected either by eating uncooked intermediate hosts or by consuming vegetables containing the living third-stage larvae. The 14-3-3β protein is a cerebrospinal fluid CSF marker of neuronal damage during the development of Creutzfeldt-Jakob disease. In addition, increased 14-3-3β protein is also found in CSF from patients with a variety of neurological disorders. The goal of this study is to determine the roles of serum-CSF14-3-3β protein in patients with eosinophilic meningitis.

MethodsIn a cohort study among nine Thai laborers with eosinophilic meningitis due to eating raw snails Pomacea canaliculata, we examined the CSF weekly while patients were still hospitalized and followed up the serum for 6 months. The levels of 14-3-3β protein in CSF were analyzed by western blot and an in-house 14-3-3β enzyme-linked immunosorbent assay ELISA measurement was established and tested in an animal model of eosinophilic meningitis.

ResultsThe elevated 14-3-3β level was detected in the CSF from eight out of nine 81% patients After 2 weeks of treatment, all patients showed a declined level or cleared of 14-3-3β protein in the CSF. By developing an in-house ELISA for measurement of 14-3-3β protein, it was found that the serum 14-3-3β level was significantly increased in patients during initial visit. . This finding was consistent to the animal experiment result in which there was severe blood brain barrier damage three weeks after infection and increased 14-3-3β protein expression in the CSF and serum by western blot and in house ELISA. After treatment, the serum 14-3-3β level in meningitis patients was rapidly returned to normal threshold. There was a correlation between initial CSF 14-3-3β level with severity of headache r = 0.692, p = 0.039, CSF pleocytosis r = 0.807, p = 0.009 and eosinophilia r = 0.798, p = 0.01 in the CSF of patients with eosinophilic meningitis Spearman’s correlation test.

ConclusionsThe serum 14-3-3β concentrations may constitute a useful marker for blood brain barrier damage severity and follow up in patients with eosinophilic meningitis caused by A. cantonensis.

KeywordsAngiostrongylus cantonensis Eosinophilic meningitis 14-3-3β protein Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-97 contains supplementary material, which is available to authorized users.

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Author: Hung-Chin Tsai - Yen-Lin Huang - Yao-Shen Chen - Chuan-Min Yen - Rachel Tsai - Susan Shin-Jung Lee - Ming-Hong Tai

Source: https://link.springer.com/







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