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C. D. AUTERI ;Revista Argentina de Microbiología 2008, 40 2

Author: C. A. ROSSETTI

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Revista Argentina de Microbiología ISSN: 0325-7541 ram@aam.org.ar Asociación Argentina de Microbiología Argentina ROSSETTI, C.
D. Long-term preservation of leptospiras by liquid nitrogen Revista Argentina de Microbiología, vol.
40, núm.
2, 2008, pp.
86-88 Asociación Argentina de Microbiología Buenos Aires, Argentina Available in: http:--www.redalyc.org-articulo.oa?id=213016787003 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative 86 Revista Argentina de Microbiología (2008) 86-88 ISSN 40: 0325-7541 Revista Argentina de Microbiología (2008) 40: 86-88 INFORME BREVE Long-term preservation of leptospiras by liquid nitrogen C.
AUTERI Area de Bacteriología, Instituto de Patobiología, CICVyA – CNIA, INTA, CC25 (1712) Castelar, Prov.
Buenos Aires, Argentina *Correspondence.
E-mail: crossetti@cnia.inta.gov.ar ABSTRACT Liquid nitrogen freezing is recommended for long-term preservation of Leptospira serovars.
However, there is no standard protocol to follow for this methodology.
We herein report a simple procedure to preserve well-characterized Leptospira serovars unaltered for long-term storage in liquid nitrogen.
Forty-three (43) leptospira strains, cryoprotected with 10% (v-v) glycerol were rapidly frozen in a dry-ice methanol bath and immediately submerged in liquid-nitrogen. Viability was retained in 100%, 93% and 83% of the frozen cultures after 6, 18 and 54 months, following freezing and storage in liquid nitrogen, respectively.
Motility and agglutinability were not altered.
These results demonstrate the usefulness of this protocol for long-term storage of genus Leptospira in liquid nitrogen. Key words: Leptospira, conservation, liquid nitrogen RESUMEN Conservación de leptospiras en...

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