ATP-diphosphophydrolase activin in rat heart tissueReport as inadecuate




ATP-diphosphophydrolase activin in rat heart tissue - Download this document for free, or read online. Document in PDF available to download.

Resumen

Extracellular nucleotides interact with specific receptors on the cell surface and are locally metabolized by ecto-nucleotidases. Biochemical characterization of the ATPase and ADPase activities detected in rat heart sarcolemma, under conditions where mitochondrial ATPase and adenylate kinase were blocked, suppor:s our proposal that both activities correspond to a single enzyme, known as ATP-diphosphohydrolase or apyrase. The physiological function of this enzyme could be dephosphorylation of the nucleotides present in the interstitial heart compartment acting together with 5-nucleotidase. Both hydrolytic activities have similarities in: sarcolemma localization, bivalent metal ion dependence, optimum pH, effect of several amino acid residue modifiers, competitive inhibition of nucleotide analogs, and broad nucleoside di- and triphosphate specificity. The ATPase activity could not be separated from the ADPase either through isoelectrofocusing or electrophoresis under acid conditions.Nota general

Artículo de publicación ISI



Author: Espinosa, Victoria; - Galleguillos, M.; - Mancilla, Marta; - Garrido, J.; - Kettlun, Ana María; - Collados, L. E.; - Chayet, L.;

Source: http://repositorio.uchile.cl/



DOWNLOAD PDF




Related documents