Comparative analysis of the gonadal transcriptomes of the all-female species Poecilia formosa and its maternal ancestor Poecilia mexicanaReport as inadecuate




Comparative analysis of the gonadal transcriptomes of the all-female species Poecilia formosa and its maternal ancestor Poecilia mexicana - Download this document for free, or read online. Document in PDF available to download.

BMC Research Notes

, 7:249

Evolutionary Biology

Abstract

BackgroundThe Amazon molly, Poecilia formosa Teleostei: Poeciliinae is an unisexual, all-female species. It evolved through the hybridisation of two closely related sexual species and exhibits clonal reproduction by sperm dependent parthenogenesis or gynogenesis where the sperm of a parental species is only used to activate embryogenesis of the apomictic, diploid eggs but does not contribute genetic material to the offspring.

Here we provide and describe the first de novo assembled transcriptome of the Amazon molly in comparison with its maternal ancestor, the Atlantic molly Poecilia mexicana. The transcriptome data were produced through sequencing of single end libraries 100 bp with the Illumina sequencing technique.

Results83,504,382 reads for the Amazon molly and 81,625,840 for the Atlantic molly were assembled into 127,283 and 78,961 contigs for the Amazon molly and the Atlantic molly, respectively. 63% resp. 57% of the contigs could be annotated with gene ontology terms after sequence similarity comparisons. Furthermore, we were able to identify genes normally involved in reproduction and especially in meiosis also in the transcriptome dataset of the apomictic reproducing Amazon molly.

ConclusionsWe assembled and annotated the transcriptome of a non-model organism, the Amazon molly, without a reference genome de novo. The obtained dataset is a fundamental resource for future research in functional and expression analysis. Also, the presence of 30 meiosis-specific genes within a species where no meiosis is known to take place is remarkable and raises new questions for future research.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-249 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Ina Maria Schedina - Stefanie Hartmann - Detlef Groth - Ingo Schlupp - Ralph Tiedemann

Source: https://link.springer.com/







Related documents