Stomatin-like protein 2 is overexpressed in epithelial ovarian cancer and predicts poor patient survivalReport as inadecuate




Stomatin-like protein 2 is overexpressed in epithelial ovarian cancer and predicts poor patient survival - Download this document for free, or read online. Document in PDF available to download.

BMC Cancer

, 15:746

First Online: 20 October 2015Received: 28 December 2014Accepted: 08 October 2015DOI: 10.1186-s12885-015-1723-x

Cite this article as: Sun, F., Ding, W., He, JH. et al. BMC Cancer 2015 15: 746. doi:10.1186-s12885-015-1723-x

Abstract

BackgroundStomatin-like protein 2 SLP-2, also known as STOML2 is a stomatin homologue of uncertain function. SLP-2 overexpression has been suggested to be associated with cancer progression, resulting in adverse clinical outcomes in patients. Our study aim to investigate SLP-2 expression in epithelial ovarian cancer cells and its correlation with patient survival.

MethodsSLP-2 mRNA and protein expression levels were analysed in five epithelial ovarian cancer cell lines and normal ovarian epithelial cells using real-time PCR and western blotting analysis. SLP-2 expression was investigated in eight matched-pair samples of epithelial ovarian cancer and adjacent noncancerous tissues from the same patients. Using immunohistochemistry, we examined the protein expression of paraffin-embedded specimens from 140 patients with epithelial ovarian cancer, 20 cases with borderline ovarian tumours, 20 cases with benign ovarian tumours, and 20 cases with normal ovarian tissues. Statistical analyses were applied to evaluate the clinicopathological significance of SLP-2 expression.

ResultsSLP-2 mRNA and protein expression levels were significantly up-regulated in epithelial ovarian cancer cell lines and cancer tissues compared with normal ovarian epithelial cells and adjacent noncancerous ovarian tissues. Immunohistochemistry analysis revealed that the relative overexpression of SLP-2 was detected in 73.6 % 103-140 of the epithelial ovarian cancer specimens, 45.0 % 9-20 of the borderline ovarian specimens, 30.0 % 6-20 of the benign ovarian specimens and none of the normal ovarian specimens. SLP-2 protein expression in epithelial ovarian cancer was significantly correlated with the tumour stage P < 0.001. Epithelial ovarian cancer patients with higher SLP-2 protein expression levels had shorter progress free survival and overall survival times compared to patients with lower SLP-2 protein expression levels. Multivariate analyses showed that SLP-2 expression levels were an independent prognostic factor for survival in epithelial ovarian cancer patients.

ConclusionsSLP-2 mRNA and proteins were overexpressed in epithelial ovarian cancer tissues. SLP-2 protein overexpression was associated with advanced stage disease. Patients with higher SLP-2 protein expression had shorter progress free survival and poor overall survival times. Thus, SLP-2 protein expression was an independent prognostic factor for patients with epithelial ovarian cancer.

KeywordsSLP-2 Epithelial ovarian cancer Prognosis Biomarker AbbreviationsANTAdjacent noncancerous tissue

NOSENormal ovarian surface epithelial

OSOverall survival

PFSProgress free survival

PVDFPolyvinylidene fluoride

SLP-2Stomatin-like protein 2

TBSTTris-buffered saline with 0.1 % Tween 20

Fei Sun and Wen Ding contributed equally to this work.

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Author: Fei Sun - Wen Ding - Jie-Hua He - Xiao-Jing Wang - Ze-Biao Ma - Yan-Fang Li

Source: https://link.springer.com/







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