Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivoReport as inadecuate




Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivo - Download this document for free, or read online. Document in PDF available to download.

Reference: Geffers, I, Serth, K, Chapman, G et al., (2007). Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivo. The Journal of Cell Biology, 178 (3), 465-476.Citable link to this page:

 

Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivo

Abstract: The Notch ligands Dll1 and Dll3 are coexpressed in the presomitic mesoderm of mouse embryos. Despite their coexpression, mutations in Dll1 and Dll3 cause strikingly different defects. To determine if there is any functional equivalence, we replaced Dll1 with Dll3 in mice. Dll3 does not compensate for Dll1; DLL1 activates Notch in Drosophila wing discs, but DLL3 does not. We do not observe evidence for antagonism between DLL1 and DLL3, or repression of Notch activity in mice or Drosophila. In vitro analyses show that differences in various domains of DLL1 and DLL3 individually contribute to their biochemical nonequivalence. In contrast to endogenous DLL1 located on the surface of presomitic mesoderm cells, we find endogenous DLL3 predominantly in the Golgi apparatus. Our data demonstrate distinct in vivo functions for DLL1 and DLL3. They suggest that DLL3 does not antagonize DLL1 in the presomitic mesoderm and warrant further analyses of potential physiological functions of DLL3 in the Golgi network.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's versionNotes:Copyright © The Rockefeller University Press. The Journal of Cell Biology, Vol. 178, No. 3, July 30, 2007 465–476http://www.jcb.org/cgi/doi/10.1083/jcb.200702009

Bibliographic Details

Publisher: Rockefeller University Press

Publisher Website: http://www.rupress.org/

Journal: The Journal of Cell Biologysee more from them

Publication Website: http://jcb.rupress.org/

Issue Date: 2007

pages:465-476Identifiers

Urn: uuid:0ca1e02d-18f0-448d-b56b-1c227233c813

Source identifier: 573456

Eissn: 1540-8140

Doi: https://doi.org/10.1083/jcb.200702009

Issn: 0021-9525 Item Description

Type: Journal article;

Version: Publisher's version Tiny URL: pubs:573456

Relationships





Author: Geffers, I - - - Serth, K - - - Chapman, G - - - Jaekel, R - - - Schuster-Gossler, K - - - Cordes, R - - - Sparrow, DB - institut

Source: https://ora.ox.ac.uk/objects/uuid:0ca1e02d-18f0-448d-b56b-1c227233c813



DOWNLOAD PDF




Related documents