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Reference: Ruth Simmons, Colin Sharp, Stuart Sims et al., (2011). High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo. Journal of Infectious Diseases, 203 (10), 1378-1387.Citable link to this page:

 

High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo

Abstract: Background. Parvovirus 4 (PARV4) is a recently identified human virus that has been found in livers of patients infected with hepatitis C virus (HCV) and in bone marrow of individuals infected with human immunodeficiency virus (HIV). T cells are important in controlling viruses but may also contribute to disease pathogenesis. The interaction of PARV4 with the cellular immune system has not been described. Consequently, we investigated whether T cell responses to PARV4 could be detected in individuals exposed to blood-borne viruses.Methods. Interferon γ (INF-γ) enzyme-linked immunospot assay, intracellular cytokine staining, and a tetrameric HLA-A*0201-peptide complex were used to define the lymphocyte populations responding to PARV4 NS peptides in 88 HCV-positive and 13 HIV-positive individuals. Antibody responses were tested using a recently developed PARV4 enzyme-linked immunosorbent assay.Results. High-frequency T cell responses against multiple PARV4 NS peptides and antibodies were observed in 26% of individuals. Typical responses to the NS pools were > 1000 spot-forming units per million peripheral blood mononuclear cells.Conclusions. PARV4 infection is common in individuals exposed to blood-borne viruses and elicits strong T cell responses, a feature typically associated with persistent, contained infections such as cytomegalovirus. Persistence of PARV4 viral antigen in tissue from HVC-positive and HIV-positive individuals and/or the associated antiviral T cell response may contribute to disease pathogenesis.

Publication status:PublishedPeer Review status:Peer reviewedVersion:Publisher's version Funder: Wellcome Trust   Funder: National Institutes of Health   Funder: Medical Research Council, UK   Notes:This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/byc/3.0), which permits unrestricted nonommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Bibliographic Details

Publisher: Oxford University Press

Publisher Website: http://www.oup.com

Host: Journal of Infectious Diseasessee more from them

Publication Website: http://jid.oxfordjournals.org/

Issue Date: 2011

Copyright Date: 2011

pages:1378-1387Identifiers

Doi: https://doi.org/10.1093/infdis/jir036

Issn: 0022-1899

Eissn: 1537-6613

Urn: uuid:0c85ea7b-8927-41e2-9cde-c5a7d0fd74a3 Item Description

Type: Article: post-print;

Language: en

Version: Publisher's versionKeywords: Parvovirus 4Subjects: Infectious diseases HIV/AIDS Tiny URL: ora:6443

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Author: Ruth Simmons - institutionUniversity of Oxford facultyMedical Sciences Division - Clinical Medicine,Nuffield Department of - - -

Source: https://ora.ox.ac.uk/objects/uuid:0c85ea7b-8927-41e2-9cde-c5a7d0fd74a3



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