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Reference: Tabdanov, E, Gondarenko, S, Kumari, S et al., (2015). Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells. Integrative biology : quantitative biosciences from nano to macro, 7 (10), 1272-1284.Citable link to this page:

 

Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells.

Abstract: The formation of the immunological synapse between a T cell and the antigen-presenting cell (APC) is critically dependent on actin dynamics, downstream of T cell receptor (TCR) and integrin (LFA-1) signalling. There is also accumulating evidence that mechanical forces, generated by actin polymerization and/or myosin contractility regulate T cell signalling. Because both receptor pathways are intertwined, their contributions towards the cytoskeletal organization remain elusive. Here, we identify the specific roles of TCR and LFA-1 by using a combination of micropatterning to spatially separate signalling systems and nanopillar arrays for high-precision analysis of cellular forces. We identify that Arp2/3 acts downstream of TCRs to nucleate dense actin foci but propagation of the network requires LFA-1 and the formin FHOD1. LFA-1 adhesion enhances actomyosin forces, which in turn modulate actin assembly downstream of the TCR. Together our data shows a mechanically cooperative system through which ligands presented by an APC modulate T cell activation.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's versionNotes:This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Bibliographic Details

Publisher: Royal Society of Chemistry

Publisher Website: http://pubs.rsc.org/

Journal: Integrative biology : quantitative biosciences from nano to macrosee more from them

Publication Website: http://pubs.rsc.org/en/journals/journal/ib

Issue Date: 2015-10

pages:1272-1284Identifiers

Urn: uuid:1bb827a7-524a-4826-b3c9-f7a51d26cbd1

Source identifier: 533220

Eissn: 1757-9708

Doi: https://doi.org/10.1039/c5ib00032g

Issn: 1757-9694 Item Description

Type: Journal article;

Language: eng

Version: Publisher's version Tiny URL: pubs:533220

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Author: Tabdanov, E - - - Gondarenko, S - - - Kumari, S - - - Liapis, A - - - Dustin, ML - institutionUniversity of Oxford Oxford, MSD, N

Source: https://ora.ox.ac.uk/objects/uuid:1bb827a7-524a-4826-b3c9-f7a51d26cbd1



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