Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes.Report as inadecuate




Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes. - Download this document for free, or read online. Document in PDF available to download.

Reference: Politis, A, Park, AY, Hyung, SJ et al., (2010). Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes. PloS one, 5 (8), e12080.Citable link to this page:

 

Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes.

Abstract: Current challenges in the field of structural genomics point to the need for new tools and technologies for obtaining structures of macromolecular protein complexes. Here, we present an integrative computational method that uses molecular modelling, ion mobility-mass spectrometry (IM-MS) and incomplete atomic structures, usually from X-ray crystallography, to generate models of the subunit architecture of protein complexes. We begin by analyzing protein complexes using IM-MS, and by taking measurements of both intact complexes and sub-complexes that are generated in solution. We then examine available high resolution structural data and use a suite of computational methods to account for missing residues at the subunit and/or domain level. High-order complexes and sub-complexes are then constructed that conform to distance and connectivity constraints imposed by IM-MS data. We illustrate our method by applying it to multimeric protein complexes within the Escherichia coli replisome: the sliding clamp, (beta2), the gamma complex (gamma3deltadelta'), the DnaB helicase (DnaB6) and the Single-Stranded Binding Protein (SSB4).

Publication status:Published

Bibliographic Details

Journal: PloS onesee more from them

Issue Date: 2010

pages:e12080Identifiers

Urn: uuid:1bcf02bc-5b23-4397-abf3-4670d9435316

Source identifier: 67460

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0012080

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng Keywords: Escherichia coli Proteins DNA-Binding Proteins Protein Subunits Crystallography, X-Ray Protein Structure, Quaternary Mass Spectrometry DnaB Helicases Computer Simulation Protein Multimerization Models, Molecular Tiny URL: pubs:67460

Relationships





Author: Politis, A - - - Park, AY - - - Hyung, SJ - - - Barsky, D - - - Ruotolo, BT - - - Robinson, CV - - - - Bibliographic Details Jour

Source: https://ora.ox.ac.uk/objects/uuid:1bcf02bc-5b23-4397-abf3-4670d9435316



DOWNLOAD PDF




Related documents