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Reference: Town, J, Pais, H, Harrison, S et al., (2016). Exploring the surfaceome of Ewing sarcoma identifies a new and unique therapeutic target. Proceedings of the National Academy of Sciences of the United States of America.Citable link to this page:

 

Exploring the surfaceome of Ewing sarcoma identifies a new and unique therapeutic target.

Abstract: The cell surface proteome of tumors mediates the interface between the transformed cells and the general microenvironment, including interactions with stromal cells in the tumor niche and immune cells such as T cells. In addition, the cell surface proteome of individual cancers defines biomarkers for thattumor type and potential proteins that can be the target of antibody-mediated therapy. We have used next-generation deep RNA sequencing (RNA-seq) coupled to an inhouse database of genes encoding cell surface proteins (herein referred to as the surfaceome) as a tool to define a cell surface proteome of Ewing sarcoma compared with progenitor mesenchymal stem cells. This subtractive RNA-seq analysis revealed a specific surfaceome of Ewing and showed unexpectedly that the leucine-rich repeat and Ig domain protein 1 (LINGO1) is expressed in over 90% of Ewing sarcoma tumors, but not expressed in any other somatic tissue apart from the brain. We found that the LINGO1 protein acts as a gateway protein internalizing into the tumor cells when engaged by antibody and can carry antibody conjugated with drugs to kill Ewing sarcoma cells. Therefore, LINGO1 is a new, unique, and specific biomarker and drug target for the treatment of Ewing sarcoma.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Medical Research Council   Funder: Wellcome Trust   Funder: Leukaemia and Lymphoma Research   Notes:Copyright © 2016 National Academy of Sciences

Bibliographic Details

Publisher: National Academy of Sciences.

Publisher Website: http://www.nasonline.org/

Journal: Proceedings of the National Academy of Sciences of the United States of Americasee more from them

Publication Website: http://www.pnas.org/

Issue Date: 2016-03Identifiers

Urn: uuid:1c27c829-8850-413f-b090-03346e599efa

Source identifier: 611286

Eissn: 1091-6490

Doi: https://doi.org/10.1073/pnas.1521251113

Issn: 0027-8424 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Ewing sarcoma LINGO1 antibody cancer cell surface Tiny URL: pubs:611286

Relationships





Author: Town, J - - - Pais, H - - - Harrison, S - - - Stead, LF - - - Bataille, C - institutionUniversity of Oxford Oxford, MPLS, Chemist

Source: https://ora.ox.ac.uk/objects/uuid:1c27c829-8850-413f-b090-03346e599efa



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