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Reference: Imwong, M, Pukrittayakamee, S, Grüner, AC et al., (2005). Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1. Malaria Journal, 4, Article: 20.Citable link to this page:

 

Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1.

Abstract: BACKGROUND: Plasmodium vivax is the second most prevalent malaria parasite affecting more than 75 million people each year, mostly in South America and Asia. In addition to major morbidity this parasite is associated with relapses and a reduction in birthweight. The emergence and spread of drug resistance in Plasmodium falciparum is a major factor in the resurgence of this parasite. P. vivax resistance to drugs has more recently emerged and monitoring the situation would be helped, as for P. falciparum, by molecular methods that can be used to characterize parasites in field studies and drug efficacy trials. METHODS: Practical PCR genotyping protocols based on polymorphic loci present in two P. vivax genetic markers, Pvcs and Pvmsp1, were developed. The methodology was evaluated using 100 P. vivax isolates collected in Thailand. RESULTS AND DISCUSSION: Analysis revealed that P. vivax populations in Thailand are highly diverse genetically, with mixed genotype infections found in 26 % of the samples (average multiplicity of infection = 1.29). A large number of distinguishable alleles were found for the two markers, 23 for Pvcs and 36 for Pvmsp1. These were generally randomly distributed amongst the isolates. A total of 68 distinct genotypes could be enumerated in the 74 isolates with a multiplicity of infection of 1. CONCLUSION: These results indicate that the genotyping protocols presented can be useful in the assessment of in vivo drug efficacy clinical trials conducted in endemic areas and for epidemiological studies of P. vivax infections.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Wellcome Trust   Notes:© 2005 Imwong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Bibliographic Details

Publisher: BioMed Central Ltd.

Publisher Website: http://www.biomedcentral.com/

Journal: Malaria Journalsee more from them

Publication Website: http://www.malariajournal.com/

Issue Date: 2005

pages:Article: 20Identifiers

Urn: uuid:36f7c56f-fbc4-4274-a41c-91acd8e49acc

Source identifier: 39505

Eissn: 1475-2875

Doi: https://doi.org/10.1186/1475-2875-4-20

Issn: 1475-2875 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Animals Humans Plasmodium vivax DNA Primers DNA, Protozoan Protozoan Proteins Merozoite Surface Protein 1 Sensitivity and Specificity Polymerase Chain Reaction Sequence Alignment Gene Frequency Gene Order Genotype Amino Acid Sequence Molecular Sequence Data Alleles Thailand Tiny URL: pubs:39505

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Author: Imwong, M - institutionUniversity of Oxford Oxford, MSD, Clinical Medicine, Thailand-Laos MOP - - - Pukrittayakamee, S - - - Grü

Source: https://ora.ox.ac.uk/objects/uuid:36f7c56f-fbc4-4274-a41c-91acd8e49acc



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